Next Article in Journal
New Insights into the Alveolar Epithelium as a Driver of Acute Respiratory Distress Syndrome
Next Article in Special Issue
Exploring Computational Data Amplification and Imputation for the Discovery of Type 1 Diabetes (T1D) Biomarkers from Limited Human Datasets
Previous Article in Journal
The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development
Previous Article in Special Issue
Metabolomic Analysis of Human Astrocytes in Lipotoxic Condition: Potential Biomarker Identification by Machine Learning Modeling
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 12
Issue 9
10.3390/biom12091270
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice

by
Qishun Zhou
1,†,
Fangrong Zhang
1,2,†,
Jakob Kerbl-Knapp
1,
Melanie Korbelius
3,
Katharina Barbara Kuentzel
3,
Nemanja Vujić
3,
Alena Akhmetshina
3,
Gerd Hörl
4,
Margret Paar
4,
Ernst Steyrer
3,
Dagmar Kratky
3,5 and
Tobias Madl
1,5,*
1
Research Unit Integrative Structural Biology, Division of Molecular Biology and Biochemistry, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
2
Key Laboratory of Gastrointestinal Cancer, Fujian Medical University, Ministry of Education, Fuzhou 350122, China
3
Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria
4
Otto-Loewi Research Center, Division of Medicinal Chemistry, Medical University of Graz, 8010 Graz, Austria
5
BioTechMed-Graz, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2022, 12(9), 1270; https://doi.org/10.3390/biom12091270
Submission received: 5 August 2022 / Revised: 2 September 2022 / Accepted: 6 September 2022 / Published: 9 September 2022
(This article belongs to the Collection Metabolomics and Integrated Multi-Omics in Health and Disease)
Browse Figures
Review Reports Versions Notes

Abstract

Phospholipid metabolism, including phosphatidylcholine (PC) biosynthesis, is crucial for various biological functions and is associated with longevity. Phosphatidylethanolamine N-methyltransferase (PEMT) is a protein that catalyzes the biosynthesis of PC, the levels of which change in various organs such as the brain and kidneys during aging. However, the role of PEMT for systemic PC supply is not fully understood. To address how PEMT affects aging-associated energy metabolism in tissues responsible for nutrient absorption, lipid storage, and energy consumption, we employed NMR-based metabolomics to study the liver, plasma, intestine (duodenum, jejunum, and ileum), brown/white adipose tissues (BAT and WAT), and skeletal muscle of young (9–10 weeks) and old (91–132 weeks) wild-type (WT) and PEMT knockout (KO) mice. We found that the effect of PEMT-knockout was tissue-specific and age-dependent. A deficiency of PEMT affected the metabolome of all tissues examined, among which the metabolome of BAT from both young and aged KO mice was dramatically changed in comparison to the WT mice, whereas the metabolome of the jejunum was only slightly affected. As for aging, the absence of PEMT increased the divergence of the metabolome during the aging of the liver, WAT, duodenum, and ileum and decreased the impact on skeletal muscle. Overall, our results suggest that PEMT plays a previously underexplored, critical role in both aging and energy metabolism.
Keywords: metabolomics; NMR; PEMT; knockout; aging; mice; liver; intestine; white/brown adipose tissue metabolomics; NMR; PEMT; knockout; aging; mice; liver; intestine; white/brown adipose tissue

Share and Cite

MDPI and ACS Style

Zhou, Q.; Zhang, F.; Kerbl-Knapp, J.; Korbelius, M.; Kuentzel, K.B.; Vujić, N.; Akhmetshina, A.; Hörl, G.; Paar, M.; Steyrer, E.; et al. Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice. Biomolecules 2022, 12, 1270. https://doi.org/10.3390/biom12091270

AMA Style

Zhou Q, Zhang F, Kerbl-Knapp J, Korbelius M, Kuentzel KB, Vujić N, Akhmetshina A, Hörl G, Paar M, Steyrer E, et al. Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice. Biomolecules. 2022; 12(9):1270. https://doi.org/10.3390/biom12091270

Chicago/Turabian Style

Zhou, Qishun, Fangrong Zhang, Jakob Kerbl-Knapp, Melanie Korbelius, Katharina Barbara Kuentzel, Nemanja Vujić, Alena Akhmetshina, Gerd Hörl, Margret Paar, Ernst Steyrer, and et al. 2022. "Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice" Biomolecules 12, no. 9: 1270. https://doi.org/10.3390/biom12091270

APA Style

Zhou, Q., Zhang, F., Kerbl-Knapp, J., Korbelius, M., Kuentzel, K. B., Vujić, N., Akhmetshina, A., Hörl, G., Paar, M., Steyrer, E., Kratky, D., & Madl, T. (2022). Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice. Biomolecules, 12(9), 1270. https://doi.org/10.3390/biom12091270

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop