Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction

Boncheva, Viktoriya B.; Linnebacher, Michael; Kdimati, Said; Draper, Hannah; Orchard, Laurence; Mills, Ken I.; O’Sullivan, Gerald; Tangney, Mark; Guinn, Barbara-ann

doi:10.3390/biom12081058

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Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction

Viktoriya B. Boncheva

^1,†

³,

⁴,

⁵

, Gerald O’Sullivan^6,‡,

Mark Tangney

^6,7,8

and

Barbara-ann Guinn

^9,*

Department of Life Sciences, University of Bedfordshire, Park Square, Luton LU1 3JU, UK

Department of General Surgery, Molecular Oncology and Immunotherapy, University Medical Center Rostock, Schillingallee 69, 18057 Rostock, Germany

Hull York Medical School, University of Hull, Cottingham Road, Kingston-upon-Hull HU6 7RX, UK

⁴

Cancer Sciences, Southampton University Hospitals Trust, University of Southampton, Southampton SO16 6YD, UK

⁵

Patrick G. Johnson Centre for Cancer Research, Queen’s University Belfast, Lisburn Road, Belfast BT9 7AE, UK

⁶

Cancer [email protected], Cork, Ireland

⁷

SynBioCentre, University College Cork, Cork, Ireland

⁸

APC Microbiome Ireland, University College Cork, Cork, Ireland

⁹

Department of Biomedical Sciences, University of Hull, Cottingham Road, Kingston-upon-Hull HU6 7RX, UK

Author to whom correspondence should be addressed.

^†

Current address: NHS Greater Glasgow and Clyde, Glasgow G12 0XH, UK.

^‡

Deceased.

Academic Editor: Alan Prem Kumar

Biomolecules 2022, 12(8), 1058; https://doi.org/10.3390/biom12081058

Received: 18 June 2022 / Revised: 21 July 2022 / Accepted: 25 July 2022 / Published: 29 July 2022

(This article belongs to the Special Issue Immunotherapy and Cancer)

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Abstract

A Crohn’s-like lymphoid reaction (CLR) is observed in about 15% of colorectal cancer (CRC) patients and is associated with favourable outcomes. To identify the immune targets recognised by CRC CLR patient sera, we immunoscreened a testes cDNA library with sera from three patients. Immunoscreening of the 18 antigens identified by SEREX with sera from normal donors showed that only the heavy chain of IgG3 (IGHG3) and a novel antigen we named UOB-COL-7, were solely recognised by sera from CRC CLR patients. ELISA showed an elevation in IgG3 levels in patients with CRC (p = 0.01). To extend our studies we analysed the expression of our SEREX-identified antigens using the RNA-sequencing dataset (GSE5206). We found that the transcript levels of multiple IGHG probesets were highly significant (p < 0.001) in their association with clinical features of CRC while above median levels of DAPK1 (p = 0.005) and below median levels of GTF2H5 (p = 0.004) and SH3RF2 (p = 0.02) were associated with improved overall survival. Our findings demonstrate the potential of SEREX-identified CRC CLR antigens to act as biomarkers for CRC and provide a rationale for their further characterization and validation. View Full-Text

Keywords: colon cancer; SEREX; crohn’s-like lymphoid reaction (CLR); immunotherapy; immunoglobulin heavy chain

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MDPI and ACS Style

Boncheva, V.B.; Linnebacher, M.; Kdimati, S.; Draper, H.; Orchard, L.; Mills, K.I.; O’Sullivan, G.; Tangney, M.; Guinn, B.-a. Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction. Biomolecules 2022, 12, 1058. https://doi.org/10.3390/biom12081058

AMA Style

Boncheva VB, Linnebacher M, Kdimati S, Draper H, Orchard L, Mills KI, O’Sullivan G, Tangney M, Guinn B-a. Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction. Biomolecules. 2022; 12(8):1058. https://doi.org/10.3390/biom12081058

Chicago/Turabian Style

Boncheva, Viktoriya B., Michael Linnebacher, Said Kdimati, Hannah Draper, Laurence Orchard, Ken I. Mills, Gerald O’Sullivan, Mark Tangney, and Barbara-ann Guinn. 2022. "Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction" Biomolecules 12, no. 8: 1058. https://doi.org/10.3390/biom12081058

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Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction

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