Gelatin-Based Electrospun Nanofibers Cross-Linked Using Horseradish Peroxidase for Plasmid DNA Delivery

Round 1

Reviewer 1 Report

1. What is the PEO content of Gelatin-Ph nanofibers in the insolubilization experiment? 2 w/v% or 4w/v%?

2. It should provide experimental evidence for the insolubility of Gelatin-Ph nanofibers, such as real-time video.

3. In addition to insoluble characterization, the authors should provide other phy-chemical characterization, such as morphology, swelling, FTIR, etc.

4. It says "The number of HEK293EGIPneo cells expressing BFP increased over days" , but the expression of BFP in figure 4d was not the highest. Please confirm it.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Review on manuscript biomolecules-1989147

The article is about the presentation of the plasmid DNA delivery using gelatin electrospun nanofibers obtained through horseradish peroxidase-mediated insolubilization. 

The article is well-written and the rsults are worth to be publish. 

I have some minor comments: 

- in the Introduction the citation of some references containing similar results must be done: e.g. 10.1002/mabi.202000040

- the purity of the applied chemicals is missing. (line 79-82)

- the description of the measuring techniques must be improved. 

- Fig. S1 contains rheology results. The concentration must be presented in the figure legend. 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

I think the present form can be accepted.