A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response

Choi, Seunga; Choi, Han-Gyu; Back, Yong Woo; Park, Hye-Soo; Lee, Kang-In; Gurmessa, Sintayehu Kebede; Pham, Thuy An; Kim, Hwa-Jung

doi:10.3390/biom11091306

Open AccessArticle

A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response

by

Seunga Choi

^1,2,†

,

Han-Gyu Choi

^1,2,†,

Yong Woo Back

^1,2,

Hye-Soo Park

^1,2,

Kang-In Lee

^1,2,

Sintayehu Kebede Gurmessa

^1,2

,

Thuy An Pham

^1,2 and

Hwa-Jung Kim

^1,2,*

¹

Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 35015, Korea

²

Department of Medical Science, College of Medicine, Chungnam National University, Daejeon 35015, Korea

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Biomolecules 2021, 11(9), 1306; https://doi.org/10.3390/biom11091306

Submission received: 14 July 2021 / Revised: 31 August 2021 / Accepted: 1 September 2021 / Published: 3 September 2021

(This article belongs to the Special Issue Virulence Factors in Mycobacterium tuberculosis Infection: Structural and Functional Studies)

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Abstract

The widely administered tuberculosis (TB) vaccine, Bacillus Calmette-Guerin (BCG), is the only licensed vaccine, but has highly variable efficiency against childhood and pulmonary TB. Therefore, the BCG prime-boost strategy is a rational solution for the development of new TB vaccines. Studies have shown that Mycobacterium tuberculosis (Mtb) culture filtrates contain proteins that have promising vaccine potential. In this study, Rv1876 bacterioferritin was identified from the culture filtrate fraction with strong immunoreactivity. Its immunobiological potential has not been reported previously. We found that recombinant Rv1876 protein induced dendritic cells’ (DCs) maturation by MAPK and NF-κB signaling activation, induced a T helper type 1 cell-immune response, and expanded the population of the effector/memory T cell. Boosting BCG with Rv1876 protein enhanced the BCG-primed Th1 immune response and reduced the bacterial load in the lung compared to those of BCG alone. Thus, Rv1876 is a good target for the prime-boost strategy.

Keywords: mycobacterium tuberculosis; dendritic cell; Rv1876; Th1 polarization; BCG-prime boost

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MDPI and ACS Style

Choi, S.; Choi, H.-G.; Back, Y.W.; Park, H.-S.; Lee, K.-I.; Gurmessa, S.K.; Pham, T.A.; Kim, H.-J. A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response. Biomolecules 2021, 11, 1306. https://doi.org/10.3390/biom11091306

AMA Style

Choi S, Choi H-G, Back YW, Park H-S, Lee K-I, Gurmessa SK, Pham TA, Kim H-J. A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response. Biomolecules. 2021; 11(9):1306. https://doi.org/10.3390/biom11091306

Chicago/Turabian Style

Choi, Seunga, Han-Gyu Choi, Yong Woo Back, Hye-Soo Park, Kang-In Lee, Sintayehu Kebede Gurmessa, Thuy An Pham, and Hwa-Jung Kim. 2021. "A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response" Biomolecules 11, no. 9: 1306. https://doi.org/10.3390/biom11091306

APA Style

Choi, S., Choi, H.-G., Back, Y. W., Park, H.-S., Lee, K.-I., Gurmessa, S. K., Pham, T. A., & Kim, H.-J. (2021). A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response. Biomolecules, 11(9), 1306. https://doi.org/10.3390/biom11091306

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A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response

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