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Article

Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements

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Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, 7624 Pecs, Hungary
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Janos Szentagothai Research Centre, University of Pecs, 20 Ifjusag Street, 7624 Pecs, Hungary
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Medical Centre, Cochrane Hungary, University of Pecs, 7623 Pecs, Hungary
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Department of Obstetrics and Gynaecology, Medical School, University of Pecs, 17 Edesanyak Street, 7624 Pecs, Hungary
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Department of Laboratory Medicine, Medical School, University of Pecs, 13 Ifjusag Street, 7624 Pecs, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Toshio Hattori
Biomolecules 2021, 11(8), 1066; https://doi.org/10.3390/biom11081066
Received: 21 June 2021 / Revised: 14 July 2021 / Accepted: 15 July 2021 / Published: 22 July 2021
Galectins are one of the critical players in the tumor microenvironment–tumor crosstalk and the regulation of local immunity. Galectin-9 has been in the limelight in tumor immunology. Galectin-9 possesses its multiplex biological functions both extracellularly and intracellularly, plays a pivotal role in the modulation of adaptive and innate immunity, and induces immune tolerance. NK-92MI cell lines against different malignancies were extensively studied, and recently published trials used genetically chimeric antigen receptor-transfected NK-92MI cells in tumor immunotherapy. Besides the intensive research in tumor immunotherapy, limited information is available on their immune-checkpoint expression and the impact of checkpoint ligands on their effector functions. To uncover the therapeutic potential of modulating Galectin-9-related immunological pathways in NK-cell-based therapy, we investigated the dose-dependent effect of soluble Galectin-9 on the TIM-3 checkpoint receptor and NKG2D, CD69, FasL, and perforin expression of NK-92MI cells. We also examined how their cytotoxicity and cytokine production was altered after Gal-9 treatment and in the presence of different serum supplements using flow cytometric analysis. Our study provides evidence that the Galectin-9/TIM-3 pathway plays an important role in the regulation of NK cell function, and about the modulatory role of Galectin-9 on the cytotoxicity and cytokine production of NK-92MI cells in the presence of different serum supplements. We hope that our results will aid the development of novel NK-cell-based strategies that target Galectin-9/TIM-3 checkpoint in tumors resistant to T-cell-based immunotherapy. View Full-Text
Keywords: NK-92MI; Galectin-9; TIM-3; cytotoxicity NK-92MI; Galectin-9; TIM-3; cytotoxicity
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MDPI and ACS Style

Meggyes, M.; Nagy, D.U.; Balassa, T.; Godony, K.; Peterfalvi, A.; Szereday, L.; Polgar, B. Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements. Biomolecules 2021, 11, 1066. https://doi.org/10.3390/biom11081066

AMA Style

Meggyes M, Nagy DU, Balassa T, Godony K, Peterfalvi A, Szereday L, Polgar B. Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements. Biomolecules. 2021; 11(8):1066. https://doi.org/10.3390/biom11081066

Chicago/Turabian Style

Meggyes, Matyas, David U. Nagy, Timea Balassa, Krisztina Godony, Agnes Peterfalvi, Laszlo Szereday, and Beata Polgar. 2021. "Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements" Biomolecules 11, no. 8: 1066. https://doi.org/10.3390/biom11081066

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