Next Article in Journal
Role of Biomolecules in Osteoclasts and Their Therapeutic Potential for Osteoporosis
Next Article in Special Issue
A Metalloproteinase Induces an Inflammatory Response in Preadipocytes with the Activation of COX Signalling Pathways and Participation of Endogenous Phospholipases A2
Previous Article in Journal
Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
Previous Article in Special Issue
Regulation of Tissue Inflammation by 12-Lipoxygenases
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 11
Issue 5
10.3390/biom11050746
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Beneficial Modulation of Lipid Mediator Biosynthesis in Innate Immune Cells by Antirheumatic Tripterygium wilfordii Glycosides

by
Kehong Zhang
1,2,†,
Simona Pace
1,†,
Paul M. Jordan
1,
Lukas K. Peltner
1,
Alexander Weber
3,
Dagmar Fischer
3,
Robert K. Hofstetter
1,
Xinchun Chen
2 and
Oliver Werz
1,*
1
Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University, Philosophenweg 14, 07743 Jena, Germany
2
Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology, Shenzhen University School of Medicine, Shenzhen 518000, China
3
Department of Chemistry and Pharmacy, Pharmaceutical Technology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Cauerstrasse 4, 91058 Erlangen, Germany
*
Author to whom correspondence should be addressed.
†
Authors contributed equally to this work.
Biomolecules 2021, 11(5), 746; https://doi.org/10.3390/biom11050746
Submission received: 14 April 2021 / Revised: 6 May 2021 / Accepted: 10 May 2021 / Published: 17 May 2021
(This article belongs to the Collection Bioactive Lipids in Inflammation, Diabetes and Cancer)
Browse Figures
Versions Notes

Abstract

Tripterygium wilfordii glycosides (TWG) is a traditional Chinese medicine with effectiveness against rheumatoid arthritis (RA), supported by numerous clinical trials. Lipid mediators (LM) are biomolecules produced from polyunsaturated fatty acids mainly by cyclooxygenases (COX) and lipoxygenases (LOX) in complex networks which regulate inflammation and immune responses and are strongly linked to RA. The mechanism by which TWG affects LM networks in RA treatment remains elusive. Employing LM metabololipidomics using ultra-performance liquid chromatography-tandem mass spectrometry revealed striking modulation of LM pathways by TWG in human monocyte-derived macrophage (MDM) phenotypes. In inflammatory M1-MDM, TWG (30 µg/mL) potently suppressed agonist-induced formation of 5-LOX products which was confirmed in human PMNL and traced back to direct inhibition of 5-LOX (IC50 = 2.9 µg/mL). TWG also efficiently blocked thromboxane formation in M1-MDM without inhibiting other prostanoids and COX enzymes. Importantly, in anti-inflammatory M2-MDM, TWG (30 µg/mL) induced pronounced formation of specialized pro-resolving mediators (SPM) and related 12/15-LOX-derived SPM precursors, without COX and 5-LOX activation. During MDM polarization, TWG (1 µg/mL) decreased the capacity to generate pro-inflammatory 5-LOX and COX products, cytokines and markers for M1 phenotypes. Together, suppression of pro-inflammatory LM but SPM induction may contribute to the antirheumatic properties of TWG.
Keywords: Tripterygium wilfordii glycosides; lipoxygenase; cyclooxygenase; inflammation; specialized pro-resolving mediators Tripterygium wilfordii glycosides; lipoxygenase; cyclooxygenase; inflammation; specialized pro-resolving mediators

Share and Cite

MDPI and ACS Style

Zhang, K.; Pace, S.; Jordan, P.M.; Peltner, L.K.; Weber, A.; Fischer, D.; Hofstetter, R.K.; Chen, X.; Werz, O. Beneficial Modulation of Lipid Mediator Biosynthesis in Innate Immune Cells by Antirheumatic Tripterygium wilfordii Glycosides. Biomolecules 2021, 11, 746. https://doi.org/10.3390/biom11050746

AMA Style

Zhang K, Pace S, Jordan PM, Peltner LK, Weber A, Fischer D, Hofstetter RK, Chen X, Werz O. Beneficial Modulation of Lipid Mediator Biosynthesis in Innate Immune Cells by Antirheumatic Tripterygium wilfordii Glycosides. Biomolecules. 2021; 11(5):746. https://doi.org/10.3390/biom11050746

Chicago/Turabian Style

Zhang, Kehong, Simona Pace, Paul M. Jordan, Lukas K. Peltner, Alexander Weber, Dagmar Fischer, Robert K. Hofstetter, Xinchun Chen, and Oliver Werz. 2021. "Beneficial Modulation of Lipid Mediator Biosynthesis in Innate Immune Cells by Antirheumatic Tripterygium wilfordii Glycosides" Biomolecules 11, no. 5: 746. https://doi.org/10.3390/biom11050746

APA Style

Zhang, K., Pace, S., Jordan, P. M., Peltner, L. K., Weber, A., Fischer, D., Hofstetter, R. K., Chen, X., & Werz, O. (2021). Beneficial Modulation of Lipid Mediator Biosynthesis in Innate Immune Cells by Antirheumatic Tripterygium wilfordii Glycosides. Biomolecules, 11(5), 746. https://doi.org/10.3390/biom11050746

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop