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Article

A Metalloproteinase Induces an Inflammatory Response in Preadipocytes with the Activation of COX Signalling Pathways and Participation of Endogenous Phospholipases A2

1
Laboratório de Farmacologia, Instituto Butantan, São Paulo 05503-900, Brazil
2
Centre of Excellence in New Target Discovery (CENTD), Instituto Butantan, São Paulo 05503-900, Brazil
3
Laboratório de Desenvolvimento e Inovação, Instituto Butantan, São Paulo 05503-900, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editors: George Kokotos and Sasanka Ramanadham
Biomolecules 2021, 11(7), 921; https://doi.org/10.3390/biom11070921
Received: 2 April 2021 / Revised: 10 June 2021 / Accepted: 11 June 2021 / Published: 22 June 2021
(This article belongs to the Collection Bioactive Lipids in Inflammation, Diabetes and Cancer)
Matrix metalloproteinases (MMPs) are proteolytic enzymes that have been associated with the pathogenesis of inflammatory diseases and obesity. Adipose tissue in turn is an active endocrine organ capable of secreting a range of proinflammatory mediators with autocrine and paracrine properties, which contribute to the inflammation of adipose tissue and adjacent tissues. However, the potential inflammatory effects of MMPs in adipose tissue cells are still unknown. This study investigates the effects of BmooMPα-I, a single-domain snake venom metalloproteinase (SVMP), in activating an inflammatory response by 3T3-L1 preadipocytes in culture, focusing on prostaglandins (PGs), cytokines, and adipocytokines biosynthesis and mechanisms involved in prostaglandin E2 (PGE2) release. The results show that BmooMPα-I induced the release of PGE2, prostaglandin I2 (PGI2), monocyte chemoattractant protein-1 (MCP-1), and adiponectin by preadipocytes. BmooMPα-I-induced PGE2 biosynthesis was dependent on group-IIA-secreted phospholipase A2 (sPLA2-IIA), cytosolic phospholipase A2-α (cPLA2-α), and cyclooxygenase (COX)-1 and -2 pathways. Moreover, BmooMPα-I upregulated COX-2 protein expression but not microsomal prostaglandin E synthase-1 (mPGES-1) expression. In addition, we demonstrate that the enzymatic activity of BmooMPα-I is essential for the activation of prostanoid synthesis pathways in preadipocytes. These data highlight preadipocytes as important targets for metalloproteinases and provide new insights into the contribution of these enzymes to the inflammation of adipose tissue and tissues adjacent to it. View Full-Text
Keywords: metalloproteinase; preadipocytes; prostaglandins; adipokines; cytokines metalloproteinase; preadipocytes; prostaglandins; adipokines; cytokines
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MDPI and ACS Style

Janovits, P.M.; Leiguez, E.; Portas, V.; Teixeira, C. A Metalloproteinase Induces an Inflammatory Response in Preadipocytes with the Activation of COX Signalling Pathways and Participation of Endogenous Phospholipases A2. Biomolecules 2021, 11, 921. https://doi.org/10.3390/biom11070921

AMA Style

Janovits PM, Leiguez E, Portas V, Teixeira C. A Metalloproteinase Induces an Inflammatory Response in Preadipocytes with the Activation of COX Signalling Pathways and Participation of Endogenous Phospholipases A2. Biomolecules. 2021; 11(7):921. https://doi.org/10.3390/biom11070921

Chicago/Turabian Style

Janovits, Priscila M., Elbio Leiguez, Viviane Portas, and Catarina Teixeira. 2021. "A Metalloproteinase Induces an Inflammatory Response in Preadipocytes with the Activation of COX Signalling Pathways and Participation of Endogenous Phospholipases A2" Biomolecules 11, no. 7: 921. https://doi.org/10.3390/biom11070921

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