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Open AccessFeature PaperArticle

DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish

1
Department of Molecular Medicine and Medical Biotechnology, University of Naples “Federico II”, Via S. Pansini 5, 80131 Naples, Italy
2
CEINGE Biotecnologie Avanzate, via Gaetano Salvatore 482, 80145 Naples, Italy
3
Department of Veterinary Medicine and Animal Productions, University of Naples “Federico II”, Via Delpino 1, 80137 Naples, Italy
4
Task Force on Microbiota Studies University of Naples “Federico II” of Naples, 80131 Naples, Italy
5
Department of Pharmacy, University “Federico II” of Naples, via Domenico Montesano, 80131 Naples, Italy
6
Department of Psychiatry and Neurobehavioural Science, APC Microbiome Institute, University College Cork, T12 YT20 Cork, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Gyeong Hoon Kang
Biomolecules 2021, 11(2), 142; https://doi.org/10.3390/biom11020142
Received: 28 December 2020 / Revised: 13 January 2021 / Accepted: 18 January 2021 / Published: 22 January 2021
(This article belongs to the Special Issue DNA Methylation Dynamics in Health and Disease)
The bidirectional microbiota–gut–brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores. View Full-Text
Keywords: DNA methylation; microbiota–gut–brain axis; Zebrafish; cell-to-cell heterogeneity; methylation profiles; epialleles DNA methylation; microbiota–gut–brain axis; Zebrafish; cell-to-cell heterogeneity; methylation profiles; epialleles
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MDPI and ACS Style

Cuomo, M.; Borrelli, L.; Della Monica, R.; Coretti, L.; De Riso, G.; D’Angelo Lancellotti di Durazzo, L.; Fioretti, A.; Lembo, F.; Dinan, T.G.; Cryan, J.F.; Cocozza, S.; Chiariotti, L. DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish. Biomolecules 2021, 11, 142. https://doi.org/10.3390/biom11020142

AMA Style

Cuomo M, Borrelli L, Della Monica R, Coretti L, De Riso G, D’Angelo Lancellotti di Durazzo L, Fioretti A, Lembo F, Dinan TG, Cryan JF, Cocozza S, Chiariotti L. DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish. Biomolecules. 2021; 11(2):142. https://doi.org/10.3390/biom11020142

Chicago/Turabian Style

Cuomo, Mariella; Borrelli, Luca; Della Monica, Rosa; Coretti, Lorena; De Riso, Giulia; D’Angelo Lancellotti di Durazzo, Luna; Fioretti, Alessandro; Lembo, Francesca; Dinan, Timothy G.; Cryan, John F.; Cocozza, Sergio; Chiariotti, Lorenzo. 2021. "DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish" Biomolecules 11, no. 2: 142. https://doi.org/10.3390/biom11020142

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