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Nephroprotective Effect of Pleurotus ostreatus and Agaricus bisporus Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak

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Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef P.O. 62521, Egypt
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Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
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Department of Zoology, Faculty of Science, El-Minia University, Minya P.O. 61519, Egypt
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Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef P.O. 62521, Egypt
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Sohag General Hospital, Sohag 42511, Egypt
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The Scientific Office of Pharma Net Egypt Pharmaceutical Company, Nasr City, Cairo 11371, Egypt
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Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(9), 1317; https://doi.org/10.3390/biom10091317
Received: 21 July 2020 / Revised: 2 September 2020 / Accepted: 5 September 2020 / Published: 14 September 2020
This study was designed to assess the nephroprotective effects of Pleurotus ostreatus and Agaricus bisporus aqueous extracts and carvedilol on hyperoxaluria-induced urolithiasis and to scrutinize the possible roles of NF-κB, p53, Bcl-2, Bax and Bak. Phytochemical screening and GC-MS analysis of mushrooms’ aqueous extracts were also performed and revealed the presence of multiple antioxidant and anti-inflammatory components. Hyperoxaluria was induced in Wistar rats through the addition of 0.75% (v/v) ethylene glycol in drinking water for nine weeks. The ethylene glycol-administered rats were orally treated with Pleurotus ostreatus and Agaricus bisporus aqueous extracts (100 mg/kg) and carvedilol (30 mg/kg) daily during the last seven weeks. The study showed that Pleurotus ostreatus, Agaricus bisporus and carvedilol all successfully inhibited ethylene glycol-induced histological perturbations and the elevation of serum creatinine, serum urea, serum and urinary uric acid, serum, urinary and kidney oxalate, urine specific gravity, kidney calcium, kidney NF-κB, NF-κB p65, NF-κB p50, p53, Bax and Bak expressions as well as serum TNF-α and IL-1β levels. Moreover, the treatment decreased the reduction in urinary creatinine, urinary urea, ratios of urinary creatinine to serum creatinine and urinary urea to serum urea, Fex Urea and Bcl-2 expression in kidney. In conclusion, although Pleurotus ostreatus and Agaricus bisporus extracts and carvedilol all significantly inhibited the progression of nephrolithiasis and showed nephroprotective effects against ethylene glycol-induced kidney dysfunction, Pleurotus ostreatus and Agaricus bisporus seemed to be more effective than carvedilol. Moreover, the nephroprotective effects may be mediated via affecting NF-κB activation, extrinsic apoptosis and intrinsic apoptosis pathways. View Full-Text
Keywords: Pleurotus ostreatus; Agaricus bisporus; carvedilol; hyperoxaluria; urolithiasis; ethylene glycol Pleurotus ostreatus; Agaricus bisporus; carvedilol; hyperoxaluria; urolithiasis; ethylene glycol
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MDPI and ACS Style

Ahmed, O.M.; Ebaid, H.; El-Nahass, E.-S.; Ragab, M.; Alhazza, I.M. Nephroprotective Effect of Pleurotus ostreatus and Agaricus bisporus Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak. Biomolecules 2020, 10, 1317. https://doi.org/10.3390/biom10091317

AMA Style

Ahmed OM, Ebaid H, El-Nahass E-S, Ragab M, Alhazza IM. Nephroprotective Effect of Pleurotus ostreatus and Agaricus bisporus Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak. Biomolecules. 2020; 10(9):1317. https://doi.org/10.3390/biom10091317

Chicago/Turabian Style

Ahmed, Osama M., Hossam Ebaid, El-Shaymaa El-Nahass, Mahmoud Ragab, and Ibrahim M. Alhazza. 2020. "Nephroprotective Effect of Pleurotus ostreatus and Agaricus bisporus Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak" Biomolecules 10, no. 9: 1317. https://doi.org/10.3390/biom10091317

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