Next Article in Journal
Hepatic Lipidomics and Molecular Imaging in a Murine Non-Alcoholic Fatty Liver Disease Model: Insights into Molecular Mechanisms
Previous Article in Journal
The Skeleton in the Closet: Faults and Strengths of Public Versus Private Genetic Biobanks
Communication

The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity

1
FONDAP Center for Genome Regulation, Faculty of Sciences, University of Chile, RM 7800003 Santiago, Chile
2
Laboratory of Systems Microbiology, Faculty of Sciences, University of Chile, RM 7800003 Santiago, Chile
3
Danio Biotechnologies SpA, RM 7800003 Santiago, Chile
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(9), 1274; https://doi.org/10.3390/biom10091274
Received: 5 July 2020 / Revised: 15 August 2020 / Accepted: 19 August 2020 / Published: 3 September 2020
In the teleost egg, the embryo is immersed in an extraembryonic fluid that fills the space between the embryo and the chorion and partially isolates it from the external environment, called the perivitelline fluid (PVF). The exact composition of the PVF remains unknown in vertebrate animals. The PVF allows the embryo to avoid dehydration, to maintain a safe osmotic balance and provides mechanical protection; however, its potential defensive properties against bacterial pathogens has not been reported. In this work, we determined the global proteomic profile of PVF in zebrafish eggs and embryos, and the maternal or zygotic origin of the identified proteins was studied. In silico analysis of PVF protein composition revealed an enrichment of protein classes associated with non-specific humoral innate immunity. We found lectins, protease inhibitors, transferrin, and glucosidases present from early embryogenesis until hatching. Finally, in vitro and in vivo experiments done with this fluid demonstrated that the PVF possessed a strong agglutinating capacity on bacterial cells and protected the embryos when challenged with the pathogenic bacteria Edwardsiella tarda. Our results suggest that the PVF is a primitive inherited immune extraembryonic system that protects the embryos from external biological threats prior to hatching. View Full-Text
Keywords: innate immunity; alpha-2-Macroglobulin; maternal immunity; lectin; chitinase; proteomics innate immunity; alpha-2-Macroglobulin; maternal immunity; lectin; chitinase; proteomics
Show Figures

Figure 1

MDPI and ACS Style

De la Paz, J.F.; Anguita-Salinas, C.; Díaz-Celis, C.; Chávez, F.P.; Allende, M.L. The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity. Biomolecules 2020, 10, 1274. https://doi.org/10.3390/biom10091274

AMA Style

De la Paz JF, Anguita-Salinas C, Díaz-Celis C, Chávez FP, Allende ML. The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity. Biomolecules. 2020; 10(9):1274. https://doi.org/10.3390/biom10091274

Chicago/Turabian Style

De la Paz, Javiera F., Consuelo Anguita-Salinas, César Díaz-Celis, Francisco P. Chávez, and Miguel L. Allende 2020. "The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity" Biomolecules 10, no. 9: 1274. https://doi.org/10.3390/biom10091274

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop