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Communication

Heparin Administered to Anopheles in Membrane Feeding Assays Blocks Plasmodium Development in the Mosquito

1
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10–12, ES-08028 Barcelona, Spain
2
Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Rosselló 149-153, ES-08036 Barcelona, Spain
3
Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-NOVA), Rua da Junqueira 100, 1349-008 Lisbon, Portugal
4
BIOIBERICA S.A.U., Polígon Industrial “Mas Puigvert”, Ctra. N-II, km. 680.6, ES-08389 Palafolls, Spain
5
Nanoscience and Nanotechnology Institute (IN2UB, Universitat de Barcelona), Martí i Franquès 1, ES-08028 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(8), 1136; https://doi.org/10.3390/biom10081136
Received: 2 June 2020 / Revised: 16 July 2020 / Accepted: 29 July 2020 / Published: 1 August 2020
Innovative antimalarial strategies are urgently needed given the alarming evolution of resistance to every single drug developed against Plasmodium parasites. The sulfated glycosaminoglycan heparin has been delivered in membrane feeding assays together with Plasmodium berghei-infected blood to Anopheles stephensi mosquitoes. The transition between ookinete and oocyst pathogen stages in the mosquito has been studied in vivo through oocyst counting in dissected insect midguts, whereas ookinete interactions with heparin have been followed ex vivo by flow cytometry. Heparin interferes with the parasite’s ookinete–oocyst transition by binding ookinetes, but it does not affect fertilization. Hypersulfated heparin is a more efficient blocker of ookinete development than native heparin, significantly reducing the number of oocysts per midgut when offered to mosquitoes at 5 µg/mL in membrane feeding assays. Direct delivery of heparin to mosquitoes might represent a new antimalarial strategy of rapid implementation, since it would not require clinical trials for its immediate deployment. View Full-Text
Keywords: malaria; heparin; mosquito; Plasmodium; Anopheles; ookinete; transmission blocking; antimalarial drugs malaria; heparin; mosquito; Plasmodium; Anopheles; ookinete; transmission blocking; antimalarial drugs
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MDPI and ACS Style

Lantero, E.; Fernandes, J.; Aláez-Versón, C.R.; Gomes, J.; Silveira, H.; Nogueira, F.; Fernàndez-Busquets, X. Heparin Administered to Anopheles in Membrane Feeding Assays Blocks Plasmodium Development in the Mosquito. Biomolecules 2020, 10, 1136. https://doi.org/10.3390/biom10081136

AMA Style

Lantero E, Fernandes J, Aláez-Versón CR, Gomes J, Silveira H, Nogueira F, Fernàndez-Busquets X. Heparin Administered to Anopheles in Membrane Feeding Assays Blocks Plasmodium Development in the Mosquito. Biomolecules. 2020; 10(8):1136. https://doi.org/10.3390/biom10081136

Chicago/Turabian Style

Lantero, Elena, Jessica Fernandes, Carlos Raúl Aláez-Versón, Joana Gomes, Henrique Silveira, Fatima Nogueira, and Xavier Fernàndez-Busquets. 2020. "Heparin Administered to Anopheles in Membrane Feeding Assays Blocks Plasmodium Development in the Mosquito" Biomolecules 10, no. 8: 1136. https://doi.org/10.3390/biom10081136

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