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Article

Enhanced Biomass Yield of and Saccharification in Transgenic Tobacco Over-Expressing β-Glucosidase

1
Bio-Energy Research Center, Chonnam National University, Gwangju 61186, Korea
2
Department of Bioenergy science and Technology, Chonnam National University, Gwangju 61186, Korea
3
Division of Business and Commerce, Chonnam National University, Yeosu 500-749, Korea
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Biomolecules 2020, 10(5), 806; https://doi.org/10.3390/biom10050806
Received: 4 May 2020 / Revised: 21 May 2020 / Accepted: 21 May 2020 / Published: 23 May 2020
(This article belongs to the Section Natural and Bio-inspired Molecules)
Here, we report an increase in biomass yield and saccharification in transgenic tobacco plants (Nicotiana tabacum L.) overexpressing thermostable β-glucosidase from Thermotoga maritima, BglB, targeted to the chloroplasts and vacuoles. The transgenic tobacco plants showed phenotypic characteristics that were significantly different from those of the wild-type plants. The biomass yield and life cycle (from germination to flowering and harvest) of the transgenic tobacco plants overexpressing BglB were 52% higher and 36% shorter than those of the wild-type tobacco plants, respectively, indicating a change in the genome transcription levels in the transgenic tobacco plants. Saccharification in biomass samples from the transgenic tobacco plants was 92% higher than that in biomass samples from the wild-type tobacco plants. The transgenic tobacco plants required a total investment (US$/year) corresponding to 52.9% of that required for the wild-type tobacco plants, but the total biomass yield (kg/year) of the transgenic tobacco plants was 43% higher than that of the wild-type tobacco plants. This approach could be applied to other plants to increase biomass yields and overproduce β-glucosidase for lignocellulose conversion. View Full-Text
Keywords: β-glucosidase; transgenic tobacco plant; biomass yield; overexpression; saccharification β-glucosidase; transgenic tobacco plant; biomass yield; overexpression; saccharification
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MDPI and ACS Style

Cho, E.J.; Nguyen, Q.A.; Lee, Y.G.; Song, Y.; Park, B.J.; Bae, H.-J. Enhanced Biomass Yield of and Saccharification in Transgenic Tobacco Over-Expressing β-Glucosidase. Biomolecules 2020, 10, 806. https://doi.org/10.3390/biom10050806

AMA Style

Cho EJ, Nguyen QA, Lee YG, Song Y, Park BJ, Bae H-J. Enhanced Biomass Yield of and Saccharification in Transgenic Tobacco Over-Expressing β-Glucosidase. Biomolecules. 2020; 10(5):806. https://doi.org/10.3390/biom10050806

Chicago/Turabian Style

Cho, Eun J., Quynh A. Nguyen, Yoon G. Lee, Younho Song, Bok J. Park, and Hyeun-Jong Bae. 2020. "Enhanced Biomass Yield of and Saccharification in Transgenic Tobacco Over-Expressing β-Glucosidase" Biomolecules 10, no. 5: 806. https://doi.org/10.3390/biom10050806

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