Intervention in Neuropsychiatric Disorders by Suppressing Inflammatory and Oxidative Stress Signal and Exploration of In Silico Studies for Potential Lead Compounds from Holigarna caustica (Dennst.) Oken leaves
by
Md. Adnan 1,†
, 
Md. Nazim Uddin Chy 2,3,†, 
A.T.M. Mostafa Kamal 2,*, Kazi Asfak Ahmed Chowdhury 2, 
Md. Atiar Rahman 4, 
A. S. M. Ali Reza 2, Md. Moniruzzaman 5, 
Satyajit Roy Rony 6, Mst. Samima Nasrin 2, 
Md. Obyedul Kalam Azad 1, Cheol Ho Park 1, Young Seok Lim 1,* and Dong Ha Cho 1,*
Md. Adnan 1,†, Md. Nazim Uddin Chy 2,3,†, A.T.M. Mostafa Kamal 2,*, Kazi Asfak Ahmed Chowdhury 2, Md. Atiar Rahman 4, A. S. M. Ali Reza 2, Md. Moniruzzaman 5, Satyajit Roy Rony 6, Mst. Samima Nasrin 2, Md. Obyedul Kalam Azad 1, Cheol Ho Park 1, Young Seok Lim 1,* and Dong Ha Cho 1,*
1
Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea
2
Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh
3
Drug Discovery, GUSTO A Research Group, Chittagong 4000, Bangladesh
4
Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh
5
Designated Reference Institute for Chemical Measurement (DRiCM), Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka 1205, Bangladesh
6
BCSIR Laboratories, Chittagong, Bangladesh Council of Scientific & Industrial Research (BCSIR), Chittagong 4220, Bangladesh
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2020, 10(4), 561; https://doi.org/10.3390/biom10040561
Received: 26 February 2020 / Revised: 28 March 2020 / Accepted: 30 March 2020 / Published: 6 April 2020
(This article belongs to the Special Issue Pharmacology of Medicinal Plants)
Holigarna caustica (Dennst.), a popular plant used in folk medicine in Bangladesh, is often used by the local folk practitioner to treat a variety of chronic diseases. The present research is an attempt to find out an innovative therapeutic prospect for the management of neuropsychiatric disorders. The methanol extract of H. caustica leaves (MEHC) were utilized on various behavioral tests for assessing anxiolytic, anti-depressant, and anti-inflammatory activities. The antioxidant potentials and quantitative phytochemicals were evaluated through spectrophotometric methods. Results revealed that treatment of MEHC (200 and 400 mg/kg) significantly reduced anxiety like behaviors in mice, particularly, 400 mg/kg efficiently improved % of entries and time spent (p < 0.05) in the open arms in elevated plus maze test, whereas, superior head dipping tendency (p < 0.05) was observed in hole-board test. In contrast, mice treated with 200 mg/kg revealed better anxiolytic effect in both open field and hole-cross tests. During antidepressant evaluation, mice administrated with MEHC exhibited active behaviors (swimming and struggling) in forced swimming and tail suspension tests. In parallel, MEHC manifested a noteworthy (p < 0.001) suppression of inflammatory response induced by histamine. The MEHC also showed strong antioxidant activities in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) (IC50: 57.64 μg/mL) scavenging, H2O2 (IC50: 51.60 μg/mL) scavenging, and ferric reducing power assay. The levels of total phenol, flavonoid, flavonol, condensed tannin, and antioxidant were estimated as higher in MEHC. Moreover, 11 compounds were documented as bioactive, displayed good binding affinities to potassium channel receptor, human serotonin receptor, cyclooxygenase (COX-1 and 2), and xanthine oxidoreductase enzyme targets in molecular docking experiments. Furthermore, ADME/T and Prediction of Activity Spectra for Substances (PASS) analyses exposed their drug-likeness, nontoxic upon consumption, and likely pharmacological actions. Overall, the H. caustica is potentially bioactive as evident by in vivo, in vitro, and computational analysis. Our findings support the folkloric value of this plant, which may provide a potential source towards developing drug leads.
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Keywords:
Holigarna caustica (Dennst.); anxiolytic; antidepressant; anti-inflammatory; antioxidant; molecular docking; ADME/T; admetSAR and PASS
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MDPI and ACS Style
Adnan, M.; Chy, M.N.U.; Kamal, A.M.; Chowdhury, K.A.A.; Rahman, M.A.; Reza, A.S.M.A.; Moniruzzaman, M.; Rony, S.R.; Nasrin, M.S.; Azad, M.O.K.; Park, C.H.; Lim, Y.S.; Cho, D.H. Intervention in Neuropsychiatric Disorders by Suppressing Inflammatory and Oxidative Stress Signal and Exploration of In Silico Studies for Potential Lead Compounds from Holigarna caustica (Dennst.) Oken leaves. Biomolecules 2020, 10, 561.
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