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Open AccessArticle

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

1
CBET Research Group, Department of Zoology & Animal Cell Biology, Faculty of Science & Technology (FCT-ZTF) and Research Centre for Experimental Marine Biology & Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), EMBRC-Spain, Areatza Hiribidea 47, 48620 Plentzia, Basque Country, Spain
2
IBEA Research Group, Department of Analytical Chemistry, Faculty of Science & Technology (FCT-ZTF) and Research Centre for Experimental Marine Biology & Biotechnology (PiE-UPV/EHU, University of the Basque Country (UPV/EHU), EMBRC-Spain, Areatza Hiribidea 47, 48620 Plentzia, Basque Country, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(3), 373; https://doi.org/10.3390/biom10030373
Received: 27 January 2020 / Revised: 21 February 2020 / Accepted: 24 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
Reactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization. View Full-Text
Keywords: Mytilus galloprovincialis; ovothiol; ovothiol synthase; glutathione; oxidative stress; metabolome; oocytes; pollution biomonitoring Mytilus galloprovincialis; ovothiol; ovothiol synthase; glutathione; oxidative stress; metabolome; oocytes; pollution biomonitoring
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Diaz de Cerio, O.; Reina, L.; Squatrito, V.; Etxebarria, N.; Gonzalez-Gaya, B.; Cancio, I. Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters. Biomolecules 2020, 10, 373.

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