Hyperoxia Alters Ultrastructure and Induces Apoptosis in Leukemia Cell Lines
by
David De Bels 1,2,3,*,†, Frauke Tillmans 1,4,†
, Francis Corazza 3,5, Mariano Bizzari 6, Peter Germonpre 1,7, Peter Radermacher 4, Keziban Günce Orman 8 and Costantino Balestra 1,9
David De Bels 1,2,3,*,†, Frauke Tillmans 1,4,†, Francis Corazza 3,5, Mariano Bizzari 6, Peter Germonpre 1,7, Peter Radermacher 4, Keziban Günce Orman 8 and Costantino Balestra 1,9
1
Environmental & Occupational Physiology Laboratory, Haute Ecole Bruxelles-Brabant, 1180 Brussels, Belgium
2
Intensive Care Department, Brugmann University Hospital, 1020 Brussels, Belgium
3
Translational Research Laboratory, Université Libre de Bruxelles, 1050 Brussels, Belgium
4
Clinic of Anesthesiology, Section Anaesthesiological Pathophysiology and Process Development, University of Ulm, 89081 Ulm, Germany
5
Immunology Laboratory, Brugmann University Hospital, 1020 Brussels, Belgium
6
Experimental Medicine Llaboratory, La Sapienza University of Rome, 00185 Rome, Italy
7
Hyperbaric Centre, Queen Astrid Military Hospital, 1120 Brussels, Belgium
8
Department of Computer Engineering, Galatasaray University, Ortakoy, Istanbul 34349, Turkey
9
Motor Sciences Department, Physical Activity Tteaching Unit, Université Libre de Bruxelles (ULB), 1050 Brussels, Belgium
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this paper.
Biomolecules 2020, 10(2), 282; https://doi.org/10.3390/biom10020282 (registering DOI)
Received: 29 December 2019 / Revised: 29 January 2020 / Accepted: 11 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue Oxygen Therapy)
Oxygenation conditions are crucial for growth and tumor progression. Recent data suggests a decrease in cancer cell proliferation occurring after exposure to normobaric hyperoxia. Those changes are associated with fractal dimension. The purpose of this research was to study the impact of hyperoxia on apoptosis and morphology of leukemia cell lines. Two hematopoietic lymphoid cancer cell lines (a T-lymphoblastoid line, JURKAT and a B lymphoid line, CCRF-SB) were tested under conditions of normobaric hyperoxia (FiO2 > 60%, ± 18h) and compared to a standard group (FiO2 = 21%). We tested for apoptosis using a caspase-3 assay. Cell morphology was evaluated by cytospin, microphotography after coloration, and analysis by a fractal dimension calculation software. Our results showed that exposure of cell cultures to transient normobaric hyperoxia induced apoptosis (elevated caspase-3) as well as significant and precocious modifications in cell complexity, as highlighted by increased fractal dimensions in both cell lines. These features are associated with changes in structure (pycnotic nucleus and apoptosis) recorded by microscopic analysis. Such morphological alterations could be due to several molecular mechanisms and rearrangements in the cancer cell, leading to cell cycle inhibition and apoptosis as shown by caspase-3 activity. T cells seem less resistant to hyperoxia than B cells.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
De Bels, D.; Tillmans, F.; Corazza, F.; Bizzari, M.; Germonpre, P.; Radermacher, P.; Orman, K.G.; Balestra, C. Hyperoxia Alters Ultrastructure and Induces Apoptosis in Leukemia Cell Lines. Biomolecules 2020, 10, 282.
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