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Open AccessArticle

Super Critical Fluid Extracted Fatty Acids from Withania somnifera Seeds Repair Psoriasis-Like Skin Lesions and Attenuate Pro-Inflammatory Cytokines (TNF-α and IL-6) Release

1
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar 249 405, India
2
Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yogpeeth, Haridwar 249 405, India
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 185; https://doi.org/10.3390/biom10020185
Received: 27 December 2019 / Revised: 17 January 2020 / Accepted: 21 January 2020 / Published: 25 January 2020
(This article belongs to the Collection Pharmacology of Medicinal Plants)
(1) Background: Withania somnifera Dunal (Ashwagandha) is a widely used medicinal herb in traditional medicinal systems with extensive research on various plant parts. Surprisingly, seeds of W. somnifera have never been investigated for their therapeutic potential. (2) Methods: W. somnifera seeds were extracted for fatty acids (WSSO) using super critical fluid extraction, and was analyzed by gas chromatography. Its therapeutic potential in psoriasis-like skin etiologies was investigated using a 12-O tetradecanoyl phorbol 13-acetate (TPA)-induced psoriatic mouse model. Psoriatic inflammation along with psoriatic lesions and histopathological scores were recorded. WSSO was also tested on murine macrophage (RAW264.7), human epidermoid (A431), and monocytic (THP-1) cells, stimulated with TPA or lipo poly-saccharide (LPS) to induce pro-inflammatory cytokine (IL-6 and TNF-α) release. NFκB promoter activity was also measured by luciferase reporter assay. (3) Results: Topical application of WSSO with concurrent oral doses significantly reduced inflammation-induced edema, and repaired psoriatic lesions and associated histopathological scores. Inhibition of pro-inflammatory cytokines release was observed in WSSO-treated A431 and THP-1 cells, along with reduced NFκB expression. WSSO also inhibited reactive nitrogen species (RNS) in LPS-stimulated RAW264.7 cells. (4) Conclusion: Here we show that the fatty acids from W. somnifera seeds have strong anti-inflammatory properties, along with remarkable therapeutic potential on psoriasis-like skin etiologies.
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Keywords: fatty acids; Withania somnifera seeds; SCFE; GC-FID; inflammation; TPA-induced psoriasis; cytokines; reactive nitrogen species; NFκB; IL-6; TNF-α fatty acids; Withania somnifera seeds; SCFE; GC-FID; inflammation; TPA-induced psoriasis; cytokines; reactive nitrogen species; NFκB; IL-6; TNF-α
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MDPI and ACS Style

Balkrishna, A.; Nain, P.; Chauhan, A.; Sharma, N.; Gupta, A.; Ranjan, R.; Varshney, A. Super Critical Fluid Extracted Fatty Acids from Withania somnifera Seeds Repair Psoriasis-Like Skin Lesions and Attenuate Pro-Inflammatory Cytokines (TNF-α and IL-6) Release. Biomolecules 2020, 10, 185.

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