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Article

Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans

1
CIRIMAT, Université de Toulouse, Université Paul Sabatier, Equipe PHYPOL, 31062 Toulouse, France
2
Department of Cardiology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autonoma de Barcelona, 08193 Barcelona, Spain
3
CIBERCV, Institute of Health Carlos III, 28029 Madrid, Spain
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Institute of Biomedical Research of Barcelona (IIBB), Spanish National Research Council (CSIC), 08036 Barcelona, Spain
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Group of Lipids and Cardiovascular Pathology, Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, 08041 Barcelona, Spain
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Research Unit on BioActive Molecules (RUBAM), Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), 08034 Barcelona, Spain
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CIBEREHD Institute of Health Carlos III, 28029 Madrid, Spain
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Department of Pathology, Hospital de la Santa Creu i Sant Pau, 08041 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(11), 1471; https://doi.org/10.3390/biom10111471
Received: 16 September 2020 / Revised: 14 October 2020 / Accepted: 21 October 2020 / Published: 22 October 2020
(This article belongs to the Special Issue Molecular Biomarkers In Cardiology)
Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography–high-resolution mass spectrometry, LC–HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans. View Full-Text
Keywords: biophysical markers; cardiac remodeling post-MI; lipidomics; Fourier transform infrared spectroscopy; heart failure biophysical markers; cardiac remodeling post-MI; lipidomics; Fourier transform infrared spectroscopy; heart failure
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MDPI and ACS Style

Samouillan, V.; Martinez de Lejarza Samper, I.M.; Benitez Amaro, A.; Vilades, D.; Dandurand, J.; Casas, J.; Jorge, E.; de Gonzalo Calvo, D.; Gallardo, A.; Lerma, E.; Guerra, J.M.; Carreras, F.; Leta, R.; Llorente Cortes, V. Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans. Biomolecules 2020, 10, 1471. https://doi.org/10.3390/biom10111471

AMA Style

Samouillan V, Martinez de Lejarza Samper IM, Benitez Amaro A, Vilades D, Dandurand J, Casas J, Jorge E, de Gonzalo Calvo D, Gallardo A, Lerma E, Guerra JM, Carreras F, Leta R, Llorente Cortes V. Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans. Biomolecules. 2020; 10(11):1471. https://doi.org/10.3390/biom10111471

Chicago/Turabian Style

Samouillan, Valerie, Ignacio M. Martinez de Lejarza Samper, Aleyda Benitez Amaro, David Vilades, Jany Dandurand, Josefina Casas, Esther Jorge, David de Gonzalo Calvo, Alberto Gallardo, Enrique Lerma, Jose M. Guerra, Francesc Carreras, Ruben Leta, and Vicenta Llorente Cortes. 2020. "Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans" Biomolecules 10, no. 11: 1471. https://doi.org/10.3390/biom10111471

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