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Intracellular Staphylococcus aureus Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity

1
Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK
2
Division of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0HS, UK
*
Authors to whom correspondence should be addressed.
Present Address: The Francis-Crick Institute, London NW1 1ST, UK.
Metabolites 2019, 9(7), 148; https://doi.org/10.3390/metabo9070148
Received: 17 June 2019 / Revised: 10 July 2019 / Accepted: 17 July 2019 / Published: 20 July 2019
(This article belongs to the Special Issue Metabolic Profiling of Cystic Fibrosis Pathogens)
As a facultative intracellular pathogen, Staphylococcus aureus is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection. Here, we investigated how S. aureus invasion affects the host membrane-bound fatty acids. We quantified the relative levels of fatty acids and their labelling pattern after intracellular infection by gas chromatography-mass spectrometry (GC-MS). Interestingly, we observed that the levels of three host fatty acids—docosanoic, eicosanoic and palmitic acids—were significantly increased in response to intracellular S. aureus infection. Accordingly, labelling carbon distribution was also affected in infected cells, in comparison to the uninfected control. In addition, treatment of HeLa cells with these three fatty acids showed a cytoprotective role by directly reducing S. aureus growth. View Full-Text
Keywords: intracellular infection; lipids metabolism; fatty acids; antibacterial effect; Staphylococcus aureus intracellular infection; lipids metabolism; fatty acids; antibacterial effect; Staphylococcus aureus
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MDPI and ACS Style

Bravo-Santano, N.; Ellis, J.K.; Calle, Y.; Keun, H.C.; Behrends, V.; Letek, M. Intracellular Staphylococcus aureus Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity. Metabolites 2019, 9, 148.

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