Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation

Current address: Division of Infection and Immunology, School of Medicine, Wales Heart Institute, Heath Park, Cardiff University, Cardiff, Wales CF14 4XN, UK.

Metabolites 2019, 9(3), 39; https://doi.org/10.3390/metabo9030039

Received: 16 January 2019 / Revised: 21 February 2019 / Accepted: 22 February 2019 / Published: 28 February 2019

(This article belongs to the Special Issue Metabolites from Phototrophic Prokaryotes and Algae Volume 2)

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Abstract

We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (liquid chromatography–tandem mass spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultures with a significant reduction in monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE) class concentrations for nutrient-deplete (LN) cultures. Moreover, reductions in the abundance of MGDG relative to total polar lipids versus an increase in the relative abundance of digalactosyldiacylglycerol (DGDG) were recorded in LN cultures. In HN cultures, polar lipid species composition remained relatively constant throughout culture with high degrees of unsaturation associated with acyl moieties. Conversely, in LN cultures lipid species composition shifted towards greater saturation of acyl moieties. Multivariate analyses revealed that changes in the abundance of a number of species contributed to the dissimilarity between LN and HN cultures but with dominant effects from certain species, e.g., reduction in MGDG 34:7 (18:3/16:4). Results demonstrate that Chlorella sp. significantly alters its polar lipidome in response to nutrient limitation, and this is discussed in terms of physiological significance and polar lipids production for applied microalgal production systems. View Full-Text

Keywords: polar lipids; Chlorella sp.; LC-MS; nutrient limitation

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MDPI and ACS Style

White, D.A.; Rooks, P.A.; Kimmance, S.; Tait, K.; Jones, M.; Tarran, G.A.; Cook, C.; Llewellyn, C.A. Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation. Metabolites 2019, 9, 39. https://doi.org/10.3390/metabo9030039

AMA Style

White DA, Rooks PA, Kimmance S, Tait K, Jones M, Tarran GA, Cook C, Llewellyn CA. Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation. Metabolites. 2019; 9(3):39. https://doi.org/10.3390/metabo9030039

Chicago/Turabian Style

White, Daniel A., Paul A. Rooks, Susan Kimmance, Karen Tait, Mark Jones, Glen A. Tarran, Charlotte Cook, and Carole A. Llewellyn. 2019. "Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation" Metabolites 9, no. 3: 39. https://doi.org/10.3390/metabo9030039

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