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Metabolites 2019, 9(2), 29; https://doi.org/10.3390/metabo9020029

Current Developments in µMAS NMR Analysis for Metabolomics

NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 91191 Gif-sur-Yvette, France
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 26 December 2018 / Revised: 2 February 2019 / Accepted: 4 February 2019 / Published: 6 February 2019
(This article belongs to the Special Issue NMR-based Metabolomics and Its Applications Volume 2)
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Abstract

Analysis of microscopic specimens has emerged as a useful analytical application in metabolomics because of its capacity for characterizing a highly homogenous sample with a specific interest. The undeviating analysis helps to unfold the hidden activities in a bulk specimen and contributes to the understanding of the fundamental metabolisms in life. In NMR spectroscopy, micro(µ)-probe technology is well-established and -adopted to the microscopic level of biofluids. However, this is quite the contrary with specimens such as tissue, cell and organism. This is due to the substantial difficulty of developing a sufficient µ-size magic-angle spinning (MAS) probe for sub-milligram specimens with the capability of high-quality data acquisition. It was not until 2012; a µMAS probe had emerged and shown promises to µg analysis; since, a continuous advancement has been made striving for the possibility of µMAS to be an effective NMR spectroscopic analysis. Herein, the mini-review highlights the progress of µMAS development—from an impossible scenario to an attainable solution—and describes a few demonstrative metabolic profiling studies. The review will also discuss the current challenges in µMAS NMR analysis and its potential to metabolomics. View Full-Text
Keywords: NMR; metabolomics; magic-angle spinning; HR-MAS; micro-MAS; metabolic profiling NMR; metabolomics; magic-angle spinning; HR-MAS; micro-MAS; metabolic profiling
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lucas-Torres, C.; Wong, A. Current Developments in µMAS NMR Analysis for Metabolomics. Metabolites 2019, 9, 29.

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