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Article

Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS

1
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
Laboratory of Forensic Medicine & Toxicology, Department of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Meikle
Metabolites 2017, 7(2), 13; https://doi.org/10.3390/metabo7020013
Received: 30 December 2016 / Revised: 25 February 2017 / Accepted: 23 March 2017 / Published: 30 March 2017
(This article belongs to the Special Issue Metabolomics 2016)
The effect of endogenous interferences of serum in multi-targeted metabolite profiling HILIC-MS/MS analysis was investigated by studying different sample preparation procedures. A modified QuEChERS dispersive SPE protocol, a HybridSPE protocol, and a combination of liquid extraction with protein precipitation were compared to a simple protein precipitation. Evaluation of extraction efficiency and sample clean-up was performed for all methods. SPE sorbent materials tested were found to retain hydrophilic analytes together with endogenous interferences, thus additional elution steps were needed. Liquid extraction was not shown to minimise matrix effects. In general, it was observed that a balance should be reached in terms of recovery, efficient clean-up, and sample treatment time when a wide range of metabolites are analysed. A quick step for removing phospholipids prior to the determination of hydrophilic endogenous metabolites is required, however, based on the results from the applied methods, further studies are needed to achieve high recoveries for all metabolites. View Full-Text
Keywords: sample preparation; serum; endogenous metabolites; targeted metabolomics sample preparation; serum; endogenous metabolites; targeted metabolomics
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MDPI and ACS Style

Tsakelidou, E.; Virgiliou, C.; Valianou, L.; Gika, H.G.; Raikos, N.; Theodoridis, G. Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS. Metabolites 2017, 7, 13. https://doi.org/10.3390/metabo7020013

AMA Style

Tsakelidou E, Virgiliou C, Valianou L, Gika HG, Raikos N, Theodoridis G. Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS. Metabolites. 2017; 7(2):13. https://doi.org/10.3390/metabo7020013

Chicago/Turabian Style

Tsakelidou, Elisavet, Christina Virgiliou, Lemonia Valianou, Helen G. Gika, Nikolaos Raikos, and Georgios Theodoridis. 2017. "Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS" Metabolites 7, no. 2: 13. https://doi.org/10.3390/metabo7020013

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