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Metabolites
Volume 14
Issue 3
10.3390/metabo14030131
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Article

In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging

by
Xue Xiao
1,2,
Xiaokang Guan
3,
Zhouyi Xu
4,* and
Qiao Lu
1,2,*
1
Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
2
Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan 442000, China
3
Discipline of Intelligent Instruments and Equipment, Xiamen University, Xiamen 361005, China
4
Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, Xiamen 361005, China
*
Authors to whom correspondence should be addressed.
Metabolites 2024, 14(3), 131; https://doi.org/10.3390/metabo14030131
Submission received: 23 January 2024 / Revised: 9 February 2024 / Accepted: 10 February 2024 / Published: 21 February 2024
(This article belongs to the Special Issue Mass Spectrometry Imaging: Theory, Methods and Applications in Biochemical and Pharmaceutical Research)
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Abstract

With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics.
Keywords: in-situ metabolic profiling; laser desorption; dielectric barrier discharge ionization; mass spectrometry imaging in-situ metabolic profiling; laser desorption; dielectric barrier discharge ionization; mass spectrometry imaging

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MDPI and ACS Style

Xiao, X.; Guan, X.; Xu, Z.; Lu, Q. In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging. Metabolites 2024, 14, 131. https://doi.org/10.3390/metabo14030131

AMA Style

Xiao X, Guan X, Xu Z, Lu Q. In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging. Metabolites. 2024; 14(3):131. https://doi.org/10.3390/metabo14030131

Chicago/Turabian Style

Xiao, Xue, Xiaokang Guan, Zhouyi Xu, and Qiao Lu. 2024. "In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging" Metabolites 14, no. 3: 131. https://doi.org/10.3390/metabo14030131

APA Style

Xiao, X., Guan, X., Xu, Z., & Lu, Q. (2024). In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging. Metabolites, 14(3), 131. https://doi.org/10.3390/metabo14030131

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