Neonatal Orally Administered Zingerone Attenuates Alcohol-Induced Fatty Liver Disease in Experimental Rat Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Setting and Animal Use Ethical Clearance
2.2. Animals and Animal Management
2.3. Experimental Design
2.4. Terminal Procedure
2.5. Computation of the Hepatosomatic Index
2.6. Determination of Hepatic Lipid Peroxidation
2.6.1. Liver Tissue Homogenisation
2.6.2. Determination of Peroxidation in the Liver
2.7. Determination of Liver Lipid Content
2.8. Determination of Liver Histomorphometry
2.9. Determination of Surrogate Plasma Biomarkers of Liver Function
2.10. Determination of Plasma CYP2E1, TNF-α and IL-6 Concentration
2.11. Determination of Hepatic Gene Expression
2.11.1. RNA Extraction and cDNA Synthesis
2.11.2. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Analysis
2.11.3. Statistical Analysis
3. Results
3.1. Effect of Neonatal Orally Administered Zingerone on Ethanol Consumption in Adult Rats
3.2. Effect of Neonatal Orally Administered Zingerone on Hepatosomatic Index
3.3. Effect of Neonatal Orally Administered Zingerone on Liver Fat Content
3.4. Effect of Neonatal Orally Administered Zingerone on Hepatic Histomorphometric Changes
3.5. Effect of Neonatal Orally Administered Zingerone on Lipid Regulatory Genes
3.6. Effect of Neonatal Orally Administered Zingerone on Plasma Liver Enzyme Activities
3.7. Effect of Neonatal Orally Administered Zingerone on Plasma CYP2E1 and Hepatic TBARS
3.8. Effect of Neonatal Orally Administered Zingerone on Biomarkers of Inflammation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment Group | Males | Females | ||
---|---|---|---|---|
Liver Mass (g) | Hepatosomatic Index (%) | Liver Mass (g) | Hepatosomatic Index (%) | |
NM + Wad | 14.19 ± 1.76 a | 2.99 ± 0.24 a | 8.06 ± 0.49 ab | 2.92 ± 0.10 a |
NM + Ethad | 13.12 ± 1.21 a | 2.96 ± 0.18 a | 7.73 ± 0.62 ab | 2.78 ± 0.12 a |
NM + Eth + Wad | 13.85 ± 2.39 a | 2.97 ± 0.25 a | 8.46 ± 0.95 a | 2.90 ± 0.18 a |
NM + Eth + Ethad | 12.76 ± 1.57 a | 2.84 ± 0.16 a | 7.56 ± 0.61 ab | 2.80 ± 0.21 a |
NM + ZO + Wad | 14.19 ± 1.76 a | 2.97 ± 0.13 a | 7.81 ± 0.98 ab | 2.82 ± 0.17 a |
NM + Eth + ZO + Wad | 13.70 ± 1.29 a | 2.94 ± 0.12 a | 8.29 ± 0.48 ab | 2.89 ± 0.18 a |
NM + ZO + Ethad | 11.73 ± 1.25 a | 2.77 ± 0.21 a | 7.28 ± 0.52 b | 2.75 ± 0.11 a |
NM + Eth + ZO + Ethad | 12.30 ± 0.95 a | 2.80 ± 0.19 a | 7.38 ± 0.41 b | 2.82 ± 0.10 a |
Treatment Group | Males | Females | ||
---|---|---|---|---|
AST(U/L) | ALT (U/L) | AST (U/L) | ALT (U/L) | |
NM + Wad | 247.8 ± 155.5 a | 61.8 ± 11.3 a | 147.2 ± 35.9 a | 57.0 ± 19.5 a |
NM + Ethad | 298.8 ± 130.4 a | 114.0 ± 47.2 a | 168.2 ± 46.6 a | 62.8 ± 22.2 a |
NM + Eth + Wad | 210.6 ± 81.8 a | 96.2 ± 36.2 a | 161.3 ± 77.0 a | 50.8 ± 19.5 a |
NM + Eth + Ethad | 156.4 ± 46.2 a | 81.0 ± 30.3 a | 200.7 ± 74.6 a | 69.5 ± 32.6 a |
NM + ZO + Wad | 183.3 ± 45.7 a | 70.3 ± 21.2 a | 145.0 ± 58.3 a | 51.8 ± 18.9 a |
NM + Eth + ZO + Wad | 224.6 ± 115.3 a | 79.8 ± 28.1 a | 121.4 ± 28.3 a | 43.0 ± 7.3 a |
NM + ZO + Ethad | 207.1 ± 102.6 a | 75.8 ± 25.8 a | 117.6 ± 14.1 a | 46.8 ± 9.4 a |
NM + Eth + ZO + Ethad | 228.7 ± 75.81 a | 95.8 ± 28.7 a | 165.0 ± 69.5 a | 46.83 ± 7.5 a |
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Asiedu, B.; Lembede, B.W.; Gomes, M.; Kasonga, A.; Nkomozepi, P.; Nyakudya, T.T.; Chivandi, E. Neonatal Orally Administered Zingerone Attenuates Alcohol-Induced Fatty Liver Disease in Experimental Rat Models. Metabolites 2023, 13, 167. https://doi.org/10.3390/metabo13020167
Asiedu B, Lembede BW, Gomes M, Kasonga A, Nkomozepi P, Nyakudya TT, Chivandi E. Neonatal Orally Administered Zingerone Attenuates Alcohol-Induced Fatty Liver Disease in Experimental Rat Models. Metabolites. 2023; 13(2):167. https://doi.org/10.3390/metabo13020167
Chicago/Turabian StyleAsiedu, Bernice, Busisani Wiseman Lembede, Monica Gomes, Abe Kasonga, Pilani Nkomozepi, Trevor Tapiwa Nyakudya, and Eliton Chivandi. 2023. "Neonatal Orally Administered Zingerone Attenuates Alcohol-Induced Fatty Liver Disease in Experimental Rat Models" Metabolites 13, no. 2: 167. https://doi.org/10.3390/metabo13020167