Effects of Solvent Evaporation Methods and Short-Term Room Temperature Storage on High-Coverage Cellular Metabolome Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Cell Culture
2.3. Sample Preparation
2.4. Chemical Isotope Labeling
2.5. LC-MS Analysis
2.6. Data Processing, Metabolite Identification, and Statistical Analysis
3. Results and Discussion
3.1. Cell Line
3.2. Metabolite Identification
3.3. Solvent Evaporation Equipment Selection
3.4. Storage Condition
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Speed * | Equipment Cost | Consumable Cost | |
---|---|---|---|
Nitrogen Blowdown Evaporator | ~an hour | ~USD 2000 | ~USD 300 per week (nitrogen gas from high pressure cylinder) or ~USD 0 (nitrogen gas from nitrogen generator) |
SpeedVac Concentrator | ~an hour | ~USD 20,000 | N/A |
Lyophilizer | ~one day | ~ USD 50,000 | N/A |
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Luo, X.; Li, L. Effects of Solvent Evaporation Methods and Short-Term Room Temperature Storage on High-Coverage Cellular Metabolome Analysis. Metabolites 2023, 13, 1052. https://doi.org/10.3390/metabo13101052
Luo X, Li L. Effects of Solvent Evaporation Methods and Short-Term Room Temperature Storage on High-Coverage Cellular Metabolome Analysis. Metabolites. 2023; 13(10):1052. https://doi.org/10.3390/metabo13101052
Chicago/Turabian StyleLuo, Xian, and Liang Li. 2023. "Effects of Solvent Evaporation Methods and Short-Term Room Temperature Storage on High-Coverage Cellular Metabolome Analysis" Metabolites 13, no. 10: 1052. https://doi.org/10.3390/metabo13101052