Stability of Wheat Floret Metabolites during Untargeted Metabolomics Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. Extract Stability at Room Temperature (25 °C)
2.1.1. Sample Reproducibility
2.1.2. Data Pre-Processing
2.2. Analytical Window Determination
3. Materials and Methods
3.1. Wheat Planting, Inoculation, and Sampling
3.2. Sample Extraction
3.3. Sample Analysis
3.4. Data Pre-Processing
3.4.1. Mass and Time Alignments
3.4.2. Recursion Analysis
3.5. Statistical Analyses
3.5.1. Blank Subtraction and Venn Diagrams
3.5.2. Interpretations
3.5.3. Principal Component Analysis
3.5.4. Volcano Plots
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Time Point | Injection Time (h:min) a |
---|---|
1 | 5:15 |
2 | 6:30 |
3 | 7:45 |
4 | 9:00 |
5 | 10:15 |
6 | 11:30 |
7 | 12:45 |
8 | 27:15 |
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Whitney, K.; Gracia-Gonzalez, G.; Simsek, S. Stability of Wheat Floret Metabolites during Untargeted Metabolomics Studies. Metabolites 2022, 12, 62. https://doi.org/10.3390/metabo12010062
Whitney K, Gracia-Gonzalez G, Simsek S. Stability of Wheat Floret Metabolites during Untargeted Metabolomics Studies. Metabolites. 2022; 12(1):62. https://doi.org/10.3390/metabo12010062
Chicago/Turabian StyleWhitney, Kristin, Gerardo Gracia-Gonzalez, and Senay Simsek. 2022. "Stability of Wheat Floret Metabolites during Untargeted Metabolomics Studies" Metabolites 12, no. 1: 62. https://doi.org/10.3390/metabo12010062