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Article

Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry

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Centre National de la Recherche Scientifique, Laboratoire d’Innovation Thérapeutique, Université de Strasbourg, CEDEX, F-67401 Illkirch, France
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Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université de Strasbourg, CEDEX, F-67084 Strasbourg, France
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Centre National de la Recherche Scientifique, Laboratoire d’Innovation Moléculaire et Applications, Université de Strasbourg-Université de Haute Alsace, CEDEX, F-67087 Strasbourg, France
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SUPINFO École des Experts des Métiers de l’Informatique, CEDEX, F-67004 Strasbourg, France
*
Author to whom correspondence should be addressed.
Academic Editor: Hirokazu Kawagishi
Metabolites 2021, 11(9), 571; https://doi.org/10.3390/metabo11090571
Received: 5 August 2021 / Revised: 16 August 2021 / Accepted: 23 August 2021 / Published: 25 August 2021
Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy, and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine (Mad), emodin (Emo), quinizarin (Qui), as well as lapachol (Lap) and fraxetin (Fra) were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoid-derived metabolites and related complexes with cations and proteins. In addition, our study validates the ability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites. These results pave the way for new hypotheses on anthranoid metabolism in plants. View Full-Text
Keywords: anthranoids; antimalarial drugs; metabolism; spectral imaging; mass spectrometry anthranoids; antimalarial drugs; metabolism; spectral imaging; mass spectrometry
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MDPI and ACS Style

Chevalier, Q.; Gallé, J.-B.; Wasser, N.; Mazan, V.; Villette, C.; Mutterer, J.; Elustondo, M.M.; Girard, N.; Elhabiri, M.; Schaller, H.; Hemmerlin, A.; Vonthron-Sénécheau, C. Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry. Metabolites 2021, 11, 571. https://doi.org/10.3390/metabo11090571

AMA Style

Chevalier Q, Gallé J-B, Wasser N, Mazan V, Villette C, Mutterer J, Elustondo MM, Girard N, Elhabiri M, Schaller H, Hemmerlin A, Vonthron-Sénécheau C. Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry. Metabolites. 2021; 11(9):571. https://doi.org/10.3390/metabo11090571

Chicago/Turabian Style

Chevalier, Quentin, Jean-Baptiste Gallé, Nicolas Wasser, Valérie Mazan, Claire Villette, Jérôme Mutterer, Maria M. Elustondo, Nicolas Girard, Mourad Elhabiri, Hubert Schaller, Andréa Hemmerlin, and Catherine Vonthron-Sénécheau. 2021. "Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry" Metabolites 11, no. 9: 571. https://doi.org/10.3390/metabo11090571

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