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Open AccessArticle

Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801

1
Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
2
Department of Biology, Washington University, St. Louis, MO 63105, USA
3
Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63105, USA
4
DBT-Pan IIT Center for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
5
Wadhwani Research Center for Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Metabolites 2020, 10(6), 250; https://doi.org/10.3390/metabo10060250
Received: 22 April 2020 / Revised: 21 May 2020 / Accepted: 12 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Metabolic Engineering and Synthetic Biology Volume 2)
Cyanobacteria are emerging as hosts for photoautotrophic production of chemicals. Recent studies have attempted to stretch the limits of photosynthetic production, typically focusing on one product at a time, possibly to minimise the additional burden of product separation. Here, we explore the simultaneous production of two products that can be easily separated: ethylene, a gaseous product, and succinate, an organic acid that accumulates in the culture medium. This was achieved by expressing a single copy of the ethylene forming enzyme (efe) under the control of PcpcB, the inducer-free super-strong promoter of phycocyanin β subunit. We chose the recently reported, fast-growing and robust cyanobacterium, Synechococcus elongatus PCC 11801, as the host strain. A stable recombinant strain was constructed using CRISPR-Cpf1 in a first report of markerless genome editing of this cyanobacterium. Under photoautotrophic conditions, the recombinant strain shows specific productivities of 338.26 and 1044.18 μmole/g dry cell weight/h for ethylene and succinate, respectively. These results compare favourably with the reported productivities for individual products in cyanobacteria that are highly engineered. Metabolome profiling and 13C labelling studies indicate carbon flux redistribution and suggest avenues for further improvement. Our results show that S. elongatus PCC 11801 is a promising candidate for metabolic engineering. View Full-Text
Keywords: cyanobacteria; metabolomics; CRISPR-Cpf1; 13C isotopic labelling; ethylene; succinate cyanobacteria; metabolomics; CRISPR-Cpf1; 13C isotopic labelling; ethylene; succinate
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MDPI and ACS Style

Sengupta, A.; Pritam, P.; Jaiswal, D.; Bandyopadhyay, A.; Pakrasi, H.B.; Wangikar, P.P. Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801. Metabolites 2020, 10, 250. https://doi.org/10.3390/metabo10060250

AMA Style

Sengupta A, Pritam P, Jaiswal D, Bandyopadhyay A, Pakrasi HB, Wangikar PP. Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801. Metabolites. 2020; 10(6):250. https://doi.org/10.3390/metabo10060250

Chicago/Turabian Style

Sengupta, Annesha; Pritam, Prem; Jaiswal, Damini; Bandyopadhyay, Anindita; Pakrasi, Himadri B.; Wangikar, Pramod P. 2020. "Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801" Metabolites 10, no. 6: 250. https://doi.org/10.3390/metabo10060250

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