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Open AccessArticle

TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro

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Department of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany
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Institute for Biostatistics and Informatics in Medicine and Ageing Research, Rostock University Medical Center, 18057 Rostock, Germany
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Department of Systems Biology and Bioinformatics, University of Rostock, 18057 Rostock, Germany
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Core Facility for Microarray Analysis, Rostock University Medical Center, 18057 Rostock, Germany
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AMP-Lab GmbH, 48149 Münster, Germany
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(2), 59; https://doi.org/10.3390/metabo10020059
Received: 24 December 2019 / Revised: 27 January 2020 / Accepted: 5 February 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Adipose Tissue and Metabolic Health)
Adipose tissue plays an active role in the regulation of the body’s energy balance. Mesenchymal stem/stromal cells from adipose tissue (adMSC) are the precursor cells for repair and adipogenesis. Since the balance of the differentiation state of adipose tissue-resident cells is associated with the development of various diseases, the examination of the regulation of proliferation and differentiation of adMSC might provide new therapeutic targets. Transforming growth factor-β1 (TGF-ß1) is synthetized by many cell types and is involved in various biological processes. Here, we investigated the effects of different concentrations of TGF-ß1 (1–10 ng/mL) on adMSC proliferation, metabolic activity, and analyzed the gene expression data obtained from DNA microarrays by bioinformatics. TGF-ß1 induced the concentration- and time-dependent increase in the cell number of adMSC with simultaneously unchanged cell cycle distributions. The basal oxygen consumption rates did not change significantly after TGF-ß1 exposure. However, glycolytic activity was significantly increased. The gene expression analysis identified 3275 differentially expressed genes upon exposure to TGF-ß1. According to the pathway enrichment analyses, they also included genes associated with energy metabolism. Thus, it was shown that TGF-ß1 induces changes in the energy metabolism of adMSC. Whether these effects are of relevance in vivo and whether they contribute to pathogenesis should be addressed in further examinations. View Full-Text
Keywords: TGF-ß1; adipose tissue-derived mesenchymal stem/stromal cells; energy metabolism; gene expression analyses TGF-ß1; adipose tissue-derived mesenchymal stem/stromal cells; energy metabolism; gene expression analyses
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Hahn, O.; Ingwersen, L.-C.; Soliman, A.; Hamed, M.; Fuellen, G.; Wolfien, M.; Scheel, J.; Wolkenhauer, O.; Koczan, D.; Kamp, G.; Peters, K. TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro. Metabolites 2020, 10, 59.

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