TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro
Abstract
1. Introduction
2. Results
2.1. Quantification of the Cell Number and Metabolic Activity
2.2. Cell Cycle Analyses
2.3. Metabolic Characterization
2.3.1. Quantification of Mitochondrial Respiration and Glycolysis
2.3.2. Gene Expression Analyses of the Energy and Amino Acid Metabolism
3. Discussion
4. Materials and Methods
4.1. Cell Isolation and Cultivation
4.2. Quantification of the Cell Number and Metabolic Activity
4.3. Cell Cycle Analyses
4.4. Quantification of Mitochondrial Respiration and Glycolysis
4.5. Gene Expression Analyses
4.5.1. RNA Extraction
4.5.2. Microarray Analysis
4.5.3. Microarray Data Analysis
4.5.4. Gene Expression Network Analysis
4.6. Data Illustration and Statistical Analysis
4.7. Ethic Statement
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
Appendix B
Appendix C
References
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Cell Cycles Phases | |||||
---|---|---|---|---|---|
sub G1 | G0/G1 | S | G2 | ||
0 d | [%] | 1.4 (± 0.1) | 89.5 (± 1.3) | 2.3 (± 0.6) | 6.1 (± 0.5) |
1 d | control [%] | 1.9 (± 0.4) | 88.7 (± 1.3) | 2.9 (± 0.3) | 6.0 (± 0.9) |
TGF-ß1 [%] | 0.9 (± 0.5) | 86.1 (± 3.9) | 5.7 (± 3.1) | 4.1 (± 1.6) | |
3 d | control [%] | 2.3 (± 0.2) | 89.3 ( ±1.0) | 2.5 (± 0.5) | 5.8 (± 0.7) |
TGF-ß1 [%] | 0.9 (± 0.3) | 89.4 (± 1.2) | 2.0 (± 0.4) | 6.3 (± 0.9) | |
7 d | control [%] | 2.5 (± 0.2) | 89.9 (± 0.8) | 2.3 (± 0.5) | 5.5 (± 0.5) |
TGF-ß1 [%] | 1.5 (± 0.2) | 87.9 (± 1.7) | 3.4 (± 1.0) | 6.2 (± 0.5) |
Energy Metabolism | Amino Acid Metabolism | ||
---|---|---|---|
Upregulated | Downregulated | Upregulated | Downregulated |
PPP3CA | PPARG | ACLY | ADH1C |
PPP3R1 | MEF2C | ALDH18A1 | ALDH1A1 |
PPP3CC | NRF1 | AOC3 | ALDH7A1 |
PPARD | PPARGC1A | ARG2 | AUH |
PRKAB2 | SIRT3 | ASNS | FAH |
PRKAG2 | TFAM | BCAT1 | GLUL |
CBS | HIBADH | ||
CTH | MCCC1 | ||
EPRS | PDK4 | ||
GCLM | PPM1L | ||
GLS | |||
GOT1 | |||
GPT2 | |||
GSR | |||
IARS | |||
ODC1 | |||
P4HA2 | |||
PYCR1 | |||
SRM | |||
WARS |
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Hahn, O.; Ingwersen, L.-C.; Soliman, A.; Hamed, M.; Fuellen, G.; Wolfien, M.; Scheel, J.; Wolkenhauer, O.; Koczan, D.; Kamp, G.; et al. TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro. Metabolites 2020, 10, 59. https://doi.org/10.3390/metabo10020059
Hahn O, Ingwersen L-C, Soliman A, Hamed M, Fuellen G, Wolfien M, Scheel J, Wolkenhauer O, Koczan D, Kamp G, et al. TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro. Metabolites. 2020; 10(2):59. https://doi.org/10.3390/metabo10020059
Chicago/Turabian StyleHahn, Olga, Lena-Christin Ingwersen, Abdelrahman Soliman, Mohamed Hamed, Georg Fuellen, Markus Wolfien, Julia Scheel, Olaf Wolkenhauer, Dirk Koczan, Günter Kamp, and et al. 2020. "TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro" Metabolites 10, no. 2: 59. https://doi.org/10.3390/metabo10020059
APA StyleHahn, O., Ingwersen, L.-C., Soliman, A., Hamed, M., Fuellen, G., Wolfien, M., Scheel, J., Wolkenhauer, O., Koczan, D., Kamp, G., & Peters, K. (2020). TGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro. Metabolites, 10(2), 59. https://doi.org/10.3390/metabo10020059