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Article

Multiplexed Quantitative Assessment of the Fate of Taurine and Sulfoquinovose in the Intestinal Microbiome

1
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, Germany
2
Research Group Intestinal Microbiology, Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, Germany
3
Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, 04103 Leipzig, Germany
*
Authors to whom correspondence should be addressed.
Metabolites 2020, 10(11), 430; https://doi.org/10.3390/metabo10110430
Received: 28 July 2020 / Revised: 13 October 2020 / Accepted: 23 October 2020 / Published: 26 October 2020
(This article belongs to the Special Issue Intercellular Metabolome)
(1) Introduction: Sulfonates, which can be diet- or host-derived, are a class of compounds detected in the gut, are involved in host–microbiome interactions and have several health effects. Our aim was to develop a method to quantify five of the sulfonates in the intestine and apply it in a simplified human microbiome model. These were taurine, its metabolic precursor cysteate and one of its degradation products isethionate, as well as sulfoquinovose and one of its most relevant degradation products 2,3-dihydroxy-1-propanesulfonate. (2) Methods: An extraction and sample preparation method was developed, without the need for derivatization. To detect and quantify the extracted sulfonates, a multiplexed LC-MS/MS-MRM method was established. (3) Results: The accuracy and precision of the method were within GLP-accepted parameters. To apply this method in a pilot study, we spiked either taurine or sulfoquinovose into an in vitro simplified human microbiota model with and without Bilophila wadsworthia, a known sulfonate utilizer. The results revealed that only the culture with B. wadsworthia was able to degrade taurine, with isethionate as an intermediate. After spiking the communities with sulfoquinovose, the results revealed that the simplified human microbiome model was able to degrade sulfoquinovose to 2,3-dihydroxypropane-1-sulfonate, which was probably catalyzed by Escherichia coli. In the community with B. wadsworthia, the 2,3-dihydroxypropane-1-sulfonate produced was further degraded by B. wadsworthia to sulfide. (4) Conclusions: We successfully developed a method for sulfonate quantification and applied it in a first pilot study. View Full-Text
Keywords: liquid chromatography; mass spectrometry; multi-reaction monitoring; quantification; sulfonates; taurine; sulfoquinovose; intestinal microbiome liquid chromatography; mass spectrometry; multi-reaction monitoring; quantification; sulfonates; taurine; sulfoquinovose; intestinal microbiome
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MDPI and ACS Style

Haange, S.-B.; Groeger, N.; Froment, J.; Rausch, T.; Burkhardt, W.; Gonnermann, S.; Braune, A.; Blaut, M.; von Bergen, M.; Rolle-Kampczyk, U. Multiplexed Quantitative Assessment of the Fate of Taurine and Sulfoquinovose in the Intestinal Microbiome. Metabolites 2020, 10, 430. https://doi.org/10.3390/metabo10110430

AMA Style

Haange S-B, Groeger N, Froment J, Rausch T, Burkhardt W, Gonnermann S, Braune A, Blaut M, von Bergen M, Rolle-Kampczyk U. Multiplexed Quantitative Assessment of the Fate of Taurine and Sulfoquinovose in the Intestinal Microbiome. Metabolites. 2020; 10(11):430. https://doi.org/10.3390/metabo10110430

Chicago/Turabian Style

Haange, Sven-Bastiaan, Nicole Groeger, Jean Froment, Theresa Rausch, Wiebke Burkhardt, Svenja Gonnermann, Annett Braune, Michael Blaut, Martin von Bergen, and Ulrike Rolle-Kampczyk. 2020. "Multiplexed Quantitative Assessment of the Fate of Taurine and Sulfoquinovose in the Intestinal Microbiome" Metabolites 10, no. 11: 430. https://doi.org/10.3390/metabo10110430

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