Next Article in Journal
Serum Metabonomic Research of the Anti-Hypertensive Effects of Ogaja on Spontaneously Hypertensive Rats
Previous Article in Journal
NMR-Based Metabolomic Analysis and Microbial Composition of Soil Supporting Burkea africana Growth
Article

HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species

1
Faculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia
2
Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia
3
Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, Slovenia
4
InnoRenew CoE, 6310 Izola, Slovenia
5
Andrej Marušič Institute, University of Primorska, 6000 Koper, Slovenia
*
Authors to whom correspondence should be addressed.
Metabolites 2020, 10(10), 403; https://doi.org/10.3390/metabo10100403
Received: 4 September 2020 / Revised: 5 October 2020 / Accepted: 8 October 2020 / Published: 12 October 2020
(This article belongs to the Section Plant Science)
Mediterranean plant Helichrysum italicum represents a rich source of versatile bioactive compounds with potential benefits for human health. Despite extensive research on the plant’s active constituents, little attention has yet been paid to characterizing the relationship between its intra-specific genetic diversity and metabolite profile. The study aimed to determine metabolic profile of H. italicum ssp. italicum (HII) and ssp. tyrrhenicum (HIT) cultivated on the experimental plantation in Slovenia and to compare the chemical composition of extracts regarding the solvent extraction process. Extracts were prepared upon conventional extract preparation procedures: maceration with 50% methanol or ethanol and cold or hot water infusion and analyzed using High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Quadrupole Time-of-Flight-Mass Spectrometry (HPLC-DAD-ESI-QTOF-MS). One hundred compounds were identified in the samples, among them several isomers and derivatives were reported for the first time, while caffeoylquinic acids and pyrones were the most abundant. Semi-quantitative comparison revealed that the extraction procedure had a greater impact on the chemical profile than genetic variability. All HIT extracts showed a higher total phenolic content compared to HII, while the antioxidant potential evaluated by 1,1-diphenyl-2-picrylhydrazil test was not proportionally higher. In addition, hot water extracts proved to be comparably active as alcoholic ones, confirming high commercial potential of Helichrysum italicum as herbal functional beverages. View Full-Text
Keywords: H. italicum; plant extracts; phenolic compounds; antioxidant activity; HPLC-DAD-ESI-QTOF-MS H. italicum; plant extracts; phenolic compounds; antioxidant activity; HPLC-DAD-ESI-QTOF-MS
Show Figures

Graphical abstract

MDPI and ACS Style

Kramberger, K.; Barlič-Maganja, D.; Bandelj, D.; Baruca Arbeiter, A.; Peeters, K.; Miklavčič Višnjevec, A.; Jenko Pražnikar, Z. HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species. Metabolites 2020, 10, 403. https://doi.org/10.3390/metabo10100403

AMA Style

Kramberger K, Barlič-Maganja D, Bandelj D, Baruca Arbeiter A, Peeters K, Miklavčič Višnjevec A, Jenko Pražnikar Z. HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species. Metabolites. 2020; 10(10):403. https://doi.org/10.3390/metabo10100403

Chicago/Turabian Style

Kramberger, Katja, Darja Barlič-Maganja, Dunja Bandelj, Alenka Baruca Arbeiter, Kelly Peeters, Ana Miklavčič Višnjevec, and Zala Jenko Pražnikar. 2020. "HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species" Metabolites 10, no. 10: 403. https://doi.org/10.3390/metabo10100403

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop