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Methods to Evaluate Skin Penetration In Vitro

Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary
Author to whom correspondence should be addressed.
Sci. Pharm. 2019, 87(3), 19;
Received: 21 May 2019 / Revised: 24 June 2019 / Accepted: 31 July 2019 / Published: 8 August 2019
(This article belongs to the Special Issue New Insights into Drug Delivery and Absorption)
PDF [1889 KB, uploaded 8 August 2019]


Dermal and transdermal drug therapy is increasing in importance nowadays in drug development. To completely utilize the potential of this administration route, it is necessary to optimize the drug release and skin penetration measurements. This review covers the most well-known and up-to-date methods for evaluating the cutaneous penetration of drugs in vitro as a supporting tool for pharmaceutical research scientists in the early stage of drug development. The aim of this article is to present various experimental models used in dermal/transdermal research and summarize the novel knowledge about the main in vitro methods available to study skin penetration. These techniques are: Diffusion cell, skin-PAMPA, tape stripping, two-photon microscopy, confocal laser scanning microscopy, and confocal Raman microscopic method.
Keywords: drug release; skin penetration; Franz cell; skin-PAMPA; tape stripping; two-photon microscopy; CLSM; confocal Raman microscopy drug release; skin penetration; Franz cell; skin-PAMPA; tape stripping; two-photon microscopy; CLSM; confocal Raman microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zsikó, S.; Csányi, E.; Kovács, A.; Budai-Szűcs, M.; Gácsi, A.; Berkó, S. Methods to Evaluate Skin Penetration In Vitro. Sci. Pharm. 2019, 87, 19.

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