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Antinociceptive Effect of Single Components Isolated from Agrimonia pilosa Ledeb. Extract

1
Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea
2
Department of Pharmacology, College of Medicine, Hallym University, Chuncheon 200-702, Korea
3
Department of Food Sciences and Nutrition, College of Natural, Health, and Life Sciences, Hallym University, Chuncheon 200-702, Korea
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2019, 87(3), 18; https://doi.org/10.3390/scipharm87030018
Received: 25 June 2019 / Revised: 26 July 2019 / Accepted: 31 July 2019 / Published: 2 August 2019
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Abstract

Agrimonia pilosa Ledeb. produces an antinociceptive effect in ICR mice in both chemically induced and thermal pain models. In the present study, we examined the antinociceptive effects of single components isolated from Agrimonia pilosa Ledeb. (AP) extract in ICR mice. Three active compounds isolated from AP, including rutin, luteolin-7-O-glucuronide, and apigenin-7-O-glucuronide, were isolated and identified by comparing EI-MS, 1H-, 13C-NMR, and UV. We studied the antinociceptive effects of three single components administered orally at doses of 10 and 20 mg/kg in monosodium urate (MSU)-treated pain model as measured by von Frey test. Among these compounds, apigenin-7-O-glucuronide was more effective in the production of antinociceptive effects. We further characterized the antinociceptive effects and possible mechanisms of apigenin-7-O-glucuronide in writhing and formalin tests. Oral administration of Apigenin-7-O-glucuronide caused a reduction in the number of writhing and effectively reduced the pain behavior observed during the second phase of the formalin test in a dose-dependent manner. In addition, the pretreatment of yohimbine instead of naloxone or methysergide attenuated apigenin-7-O-glucuronide-induced antinociception in the writhing test. Moreover, apigenin-7-O-glucuronide caused reduction in the expression of p-P38, p-CREB, and p-mTOR induced by formalin injection. Our results indicate that apigenin-7-O-glucuronide shows an antinociceptive effect in various pain models. In addition, spinal α2-adrenergic receptors appear to be involved in the production of antinociception induced by apigenin-7-O-glucuronide. Furthermore, the antinociceptive effect of apigenin-7-O-glucuronide appears to be mediated by reduction in the expression of p-P38, p-CREB and p-mTOR levels in the spinal cord. View Full-Text
Keywords: apigenin-7-O-glucuronide; antinociception; α2-adrenergic receptors; spinal signal molecules apigenin-7-O-glucuronide; antinociception; α2-adrenergic receptors; spinal signal molecules
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Feng, J.H.; Lee, H.J.; Kim, S.B.; Jung, J.S.; Lim, S.S.; Suh, H.W. Antinociceptive Effect of Single Components Isolated from Agrimonia pilosa Ledeb. Extract. Sci. Pharm. 2019, 87, 18.

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