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Article

Anti-Oxidant and Anti-Aging Activities of Callus Culture from Three Rice Varieties

1
School of Cosmetic Science, Mae Fah Luang University, 333, Moo.1, Thasud, Muang, Chiang Rai 57100, Thailand
2
Cosmetic and Beauty Innovations for Sustainable Development (CBIS) Research Group, Mae Fah Luang University, 333, Moo.1, Thasud, Muang, Chiang Rai 57100, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Enzo Berardesca
Cosmetics 2022, 9(4), 79; https://doi.org/10.3390/cosmetics9040079
Received: 23 April 2022 / Revised: 18 June 2022 / Accepted: 6 July 2022 / Published: 30 July 2022
The aims of this study were to induce calli from the seeds of three rice varieties (Hommali 105, Munpu, and Niawdum) and investigate their anti-aging potential. First, rice seeds were cultured on a Murashige and Skoog medium (MS medium) supplemented with 2 mg/L of 2,4-Dichlorophenoxyacetic acid (2,4-D), 1 mg/L of 1-Naphthalene acetic acid (NAA), and 1 mg/L of 6-Benzylaminopurine (BAP). After three weeks, the calli were extracted with ethanol. Then, their phenolic contents were determined by spectrophotometer and the amino acids were identified by ultra-performance liquid chromatography (UPLC). Their cytotoxicity, anti-oxidant (potassium ferricyanide reducing power assay (PFRAP), DPPH radical scavenging assay (DPPH), lipid peroxidation inhibition (LPO), and superoxide dismutase activity (SOD)), and anti-aging (keratinocyte proliferation, anti-collagenase, anti-inflammation, and anti-tyrosinase) activities were also investigated. Munpu callus (385%) was obtained with a higher yield than Hommali (322%) and Niawdum (297%) calli. The results revealed that the phenolic and amino acid contents were enhanced in the calli. Moreover, the calli were rich in glutamic acid, alanine, and gamma aminobutyric acid (GABA). The callus extracts showed no cytotoxic effects at a concentration of equal to or lower than 0.25 mg/mL. The highest anti-oxidant activities (PFRAP (0.81 mg AAE/mL), DPPH (68.22%), LPO (52.21%), and SOD (67.16%)) was found in Munpu callus extract. This extract also had the highest keratinocyte proliferation (43.32%), anti-collagenase (53.83%), anti-inflammation (85.40%), and anti-tyrosinase (64.77%) activities. The experimental results suggest that the amounts of bioactive compounds and anti-aging activities of rice seeds can be enhanced by the induction of callus formation. View Full-Text
Keywords: anti-aging; anti-oxidant; callus; pigmented rice; plant stem cell; rice stem cell anti-aging; anti-oxidant; callus; pigmented rice; plant stem cell; rice stem cell
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MDPI and ACS Style

Vitchit, W.; Saewan, N. Anti-Oxidant and Anti-Aging Activities of Callus Culture from Three Rice Varieties. Cosmetics 2022, 9, 79. https://doi.org/10.3390/cosmetics9040079

AMA Style

Vitchit W, Saewan N. Anti-Oxidant and Anti-Aging Activities of Callus Culture from Three Rice Varieties. Cosmetics. 2022; 9(4):79. https://doi.org/10.3390/cosmetics9040079

Chicago/Turabian Style

Vitchit, Wannisa, and Nisakorn Saewan. 2022. "Anti-Oxidant and Anti-Aging Activities of Callus Culture from Three Rice Varieties" Cosmetics 9, no. 4: 79. https://doi.org/10.3390/cosmetics9040079

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