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Review

Tyrosinase and Melanogenesis Inhibition by Indigenous African Plants: A Review

by
Laurentia Opperman
1,
Maryna De Kock
1,
Jeremy Klaasen
1 and
Farzana Rahiman
1,2,*
1
Department of Medical Bioscience, University of the Western Cape, Cape Town 7535, South Africa
2
Skin Research Lab, Department of Medical Biosciences, University of the Western Cape, Robert Sobukwe Rd, Bellville, Cape Town 7535, South Africa
*
Author to whom correspondence should be addressed.
Cosmetics 2020, 7(3), 60; https://doi.org/10.3390/cosmetics7030060
Submission received: 23 June 2020 / Revised: 7 July 2020 / Accepted: 13 July 2020 / Published: 29 July 2020
(This article belongs to the Special Issue Feature Papers in Cosmetics in 2020)
Browse Figure
Versions Notes

Abstract

:
The indiscriminate use of non-regulated skin lighteners among African populations has raised health concerns due to the negative effects associated with skin lightener toxicity. For this reason, there is a growing interest in the cosmetic development of plants and their metabolites as alternatives to available chemical-derived skin lightening formulations. Approximately 90% of Africa’s population depends on traditional medicine, and the continent’s biodiversity holds plant material with various biological activities, thus attracting considerable research interest. This study aimed to review existing evidence and document indigenous African plant species capable of inhibiting the enzyme tyrosinase and melanogenesis for potential incorporation into skin lightening products. Literature search on melanin biosynthesis, skin lightening, and tyrosinase inhibitors resulted in the identification of 35 plant species were distributed among 31 genera and 21 families across 15 African countries and 9 South African provinces. All plants identified in this study showed competent tyrosinase and melanogenesis inhibitory capabilities. These results indicate that African plants have the potential to serve as alternatives to current chemically-derived skin lighteners.

1. Introduction

Melanin is a widespread natural pigment that is responsible for color in hair, skin, and eyes. It provides protection against the deleterious effects of ultraviolet (UV) irradiation [1]. Melanogenesis is the physiological process of melanin formation in which TYR, a copper-dependent enzyme, initiates the first step. Tyrosinase catalyzes the rate-limiting step where L-tyrosine is converted to L-3,4,-dihydroxyphenylalanine (L-DOPA), leading to the eventual formation of the pigment (Illustrated by Scheme 1) [2,3,4,5]. Abnormal TYR activity leads to pigmentary disorders, such as the abnormal accumulation of melanin (hyperpigmentation) that accounts for most dermatology visits [6,7,8]. Skin lighteners can be divided by their mechanisms of action, such as inhibition of tyrosinase transcription, inhibition of melanosome transfer, and accelerated epidermal turnover, with the most common target being tyrosinase (TYR) inhibition [9,10]. By decreasing the activity and/or expression of TYR, melanogenesis can be inhibited, leading to reduced melanin production [11].
The skin lightening industry is one of the fastest-growing segments of the global beauty industry. Global industry analysts (GIA) have predicted that by 2020, the universal skin lightening market will reach $23 billion [12]. A recent meta-analysis provided evidence of the global prevalence of skin lightening use by reporting an estimate of 27.7%, with Africa at a current estimated prevalence of 27.1% [13]. Previous epidemiological studies have also reported a high prevalence of skin lightener use among African populations. This is evident among South African, Senegalese, and Nigerian study populations that revealed between 32 to 75% skin lightener use [14,15,16].
This practice is motivated by a long-standing history of social divisions, including societal pressures and stigmas, leading to the demand for lighter skin tones [17,18]. Creams, lotions, soaps, and injections indicated as a treatment for hyperpigmentation disorders are exceedingly abused as self-medication to achieve a lighter skin complexion [19,20]. In many African countries, a variety of these skin lightening preparations are easily obtained over-the-counter without a medical prescription, despite this being a requirement by law [15,21]. The most frequently used ingredients include steroids, mercury, hydroquinone (HQ) (considered the gold standard), and its derivatives [7]. Health concerns associated with the long-term use of these skin lightener ingredients include exogenous ochronosis and infectious dermatosis [22,23]. Furthermore, heavy metal exposure can lead to damage to the circulatory and urinary systems [24]. Due to their toxicity, these compounds have been prohibited as skin lightening compounds in several African countries, including South Africa, Nigeria, Kenya, and the Ivory Coast [25,26]. Despite this ban, these damaging chemicals are often illegally introduced into cosmetic formulations and, the public continues to gain access via informal channels such as street vendors, markets, and non-pharmaceutical shops [27,28]. In contrast, botanicals and natural ingredients offer safer alternatives as they may not exhibit the same kind of toxicity as synthetic compounds and could exhibit much less harmful side effects [29]. Despite this, consumers are not generally aware that natural products are composed of a variety of chemical compounds that could lead to the development of some adverse reactions. These potential effects could be overcome by researchers chemically characterizing extracts with respect to its composition [30].
Botanicals and natural ingredients provide abundant sources of treatment for various diseases such as cancer, diabetes, and dermatological conditions [31,32]. The use of plants is a common practice in traditional medicines of many cultures using several plant extracts as cosmetics to improve skin health [33,34]. This could be attributed to plant extracts being a rich source of vitamins, antioxidants, oils/essential oils, and other bioactive compounds, which provide the body with nutrients necessary for healthy skin [30]. Plants also constitute a variety of chemical compounds that elicit various pharmacological activities with the possibility that these compounds act synergistically to produce a net pharmacological effect [35]. Examples of such compounds include polyphenols and flavonoids. Polyphenols are widely distributed in plants, and several polyphenol types have been reported as being responsible for melanogenesis inhibition [36,37]. Flavonoids and chalcones are a group of polyphenols with flavonoids being one of the most explored and most numerous groups of polyphenols [38]. Flavonoids are found within the leaves, seeds, bark, and flowers of most plants, and have been studied for its oxidation of L-DOPA and have shown good antityrosinase. Furthermore, chalcones exhibit a wide array of biological activities with a number of chalcones eliciting antityrosinase activity [39,40,41].
The significant advancement of research using plant extracts in cosmetics demonstrates the growing interest of researchers and pharmaceutical companies in developing natural skin lightening products [42]. The objective of this review was to examine existing literature to identify and document indigenous African plant species capable of inhibiting the enzyme TYR and melanogenesis for possible use as alternatives to current skin lightening formulations.

2. Materials and Methods

A computerized literature search was performed using the following databases: MEDLINE, SCOPUS, GOOGLE SCHOLAR, MEDLINE EBSCOHOST, and SCIENCE DIRECT databases. In addition, the South African National Electronic Thesis Database (ETD) was searched for grey literature, which included Masters and Doctoral theses. The following key terms were used for the retrieval of articles in the databases: “skin lightening”, “tyrosinase”, “melanin”, “antimelanogenesis”, “antityrosinase”, “melanogenesis”, “tyrosinase inhibition”, “melanin inhibition”. For an article to be considered eligible, the following criteria needed to be met: (1) The use of indigenous African plant extracts (alone or in combination with other African plants); (2) performed in vivo and in vitro studies only; (3) investigated tyrosinase and melanogenesis inhibition. This literature search also had no restrictions on the following: Language; date of publication, and publication status (inclusive of published, unpublished, in the press and in progress). Studies that reported on both non-African (not indigenous to Africa and imported plants) and African plants were isolated, and only the African plants were included in this study. In addition, studies that included tests other than tyrosinase and melanin assays were isolated, and only the tyrosinase and melanin assays were reported on. All qualitative studies were excluded. Three independent reviewers completed the above-described methods independently. Any disagreements between the reviewers were discussed and resolved.
The articles that had been retrieved through the computerized literature searches were combined, giving a total of 128 articles. A preliminary analysis of the titles and abstracts of each article was performed, and all duplicates were excluded. After the screening of abstracts by at least 2 authors, the articles classified as ineligible based on the previously described criteria were excluded, and a total of 49 articles underwent a full-text review.
After further application of the exclusion and inclusion criteria, a total of 36 articles were classified as eligible for discussion in this review. Table 1 and Table 2 summarizes the plant species identified along with their melanin and tyrosinase results, respectively. In both tables, the plant names are arranged according to their family, along with the region the plants are found in Africa and plant part used.

3. Results and Discussion

In this study, 35 plant species distributed across 31 genera and 21 families were identified as being effective as TYR and melanogenesis inhibitors. In addition, the plants identified in this study were distributed among 15 African countries and 9 South African provinces. 17 (47.2%) were found in South Africa, with 19 (52.7%) found within other African countries. The most represented families were Fabaceae (5 plant species), Melianthaceae (3 plant species), Sapotaceae (3 plant species), Chenopodiaceae (2 plant species), Proteaceae (2 plant species), Clusiaceae (2 plant species), Rhizophoraceae (2 plant species), and Lamiaceae (2 plant species). The rest of the families were represented with only 1 plant species—Anacardiaceae, Apiaceae, Asteraceae, Brassiaceae, Capparaceae, Euphorbiaceae, Myrsinaceae, Pedaliaceae, Picrodendraceae, Poaceae, Podocarpaceae, Rubiaceae, and Thymelaeaceae.
African forests are the world’s second-largest tropical reservoir holding very promising plant materials with various biological activities, which has attracted considerable research interest [50]. Up to 90% of Africa’s human population depends directly on traditional medicine. Plants form a central component of the African traditional healthcare system and is probably the oldest of all therapeutic systems [71,72]. The importance of this resource can be illustrated by the comprehensive list of African medicinal plants in which more than 5400 plant taxa and over 16,300 medicinal uses for the plants have been identified. The use of plant extracts as topical treatments has been practiced for many generations with extracts being used for the treatment of various skin ailments, including wounds, skin infections, and inflammation [73,74]. The demand for cosmetic skin-lightening products is growing, with predictions particular to Asia and Africa forecasting the beauty industry to be worth an estimated $US 31.2 billion by 2024 [12,75]. This significant increase can also be accounted for by the pleasant aromatics and the general consensus that plant extracts are safer than synthetic products available. Thus, there is an ever-growing endeavour to explore plant-based melanogenesis inhibitors [76,77].
Various plant extracts and compounds have been investigated for their anti-tyrosinase and antimelanogenic effects [78]. Three methods are extensively used to study tyrosinase activity, which includes 2 radiometric assays (tyrosinase hydroxylase and melanin formation activities) and one spectrophotometric assay (dopa oxidase activity). Tyrosinase hydroxylase assay estimates the tyrosinase hydroxylase activity of tyrosinase by measuring tritiated water released from L-[3,5-3H]-tyrosine. The melanin formation activity assay estimates the radioactive melanin synthesized from L-[U-14C]-tyrosine while the dopa oxidase activity measures the rate of dopachrome formation, of which all three are in vitro assays [79]. These assays also include the use of positive controls whose potencies are well-known, such as kojic acid (KA), to which the substance of interest can be compared [80]. Results obtained from these assays are often presented in IC50 values, which refers to the concentration of plant extract at which half the original TYR activity is inhibited [40].
As shown in the results described in Table 2, plants reported from the Fabaceae family were only tested for their ability as TYR inhibitors, and all proved to be strong inhibitors. Further results obtained by Lall et al. [65], supported the findings for Ormocarpum and Acacia nilotica, which demonstrated the lowest IC50 value of 2.95 μg/mL and showed to have the highest TYR inhibition of 98.3% (IC50 8.61 μg/mL), respectively [36,63]. Cassipourea congoensis demonstrated significant effects of both crude extract and isolated compounds on melanin and TYR activity, respectively [49]. Rorippa nasturtium-aquaticum (Brassiaceae) showed in studies conducted by both Thibane et al. [59,60], that the extract is an effective TYR inhibitor (IC50 values of 22.24 and 1.513 μg/mL respectively) when compared to the kojic acid (KA) control (19.38 and 1.421 μg/mL, respectively). It is also noted that KA is the most prominent (91.7%, 33 articles) positive control used in the studies identified, due to its well-established potency in literature [80]. Arbutin, a HQ derivative, was the second most common (35.3%, 12 articles) used positive control as it is generally used in cosmetics as a hypopigmenting agent [81].
Studies on Thymelaea hirsuta (Thymelaeaceae) reported that this extract inhibited more than 50% of melanin at 1 μg/mL [53]. Furthermore, isolated compounds of this extract indicated that melanin production was reduced by 37% at 0.1 μg/mL, in comparison to its arbutin control, which only inhibited 33% of melanin at a higher concentration—100 μg/mL [54]. These results are further supported by Villareal et al. [55], who concluded that isolated compounds of Thymelaea hirsuta at 0.1 μg/mL (33% reduction of MC) is as effective as arbutin, a common depigmenting agent, at 100 μg/mL. Rhizophoraceae extracts (Cassipourea flanaganii and Cassipourea congoensis) exhibited compelling skin lightening properties with IC50 values obtained from studies conducted on Cassipourea flanaganii, which indicated values (1.425 μg/mL and 22.24 μg/mL, respectively) comparable to their KA controls (1.421 μg/mL and 19.38 μg/mL, respectively) [48,49]. Argania spinosa (Sapotaceae) effectively inhibited melanogenesis at 55% after 72 hours’ exposure [50]. These findings are supported by Villareal et al. [23], showing that there is a greater than 50% reduction in melanin content after 72 hours of exposure. Although significant results were obtained from separate studies, the difference in the result could be attributed to the researchers investigating different parts of the same plant (Table 1) as well as differences in plant preparation and assay protocols.
The plants in this study were distributed among 15 African countries with studies, including data from 9 South African provinces. Twenty plants species were investigated using aerial parts/leaves with these plants being collected in different regions of their respective countries and/or provinces. Thus, in natural ecosystems, factors affecting the plant’s performance include climate, soil, and geographic locations yielding various molecular complexes, thus, emphasizing the environment’s crucial role in the metabolism of plants [82,83].
The results obtained from the TYR and melanin assays of Harpephyllum caffrum showed the bark extract to have the highest inhibitory effect on TYR and melanin production in comparison to the leaf extract of the same species [32]. These results are further corroborated by a review conducted by Lall and Kishore. [84], where it was noted that Harpephyllum caffrum and Greyia flanaganii, among other listed plants, showed promising pharmacological activities, a finding that warrants further scientific investigation. Similar comparisons can be seen with Ceratonia siliqua concerning TYR activity where its isolated compounds (90% inhibition) were shown to be a more potent TYR inhibitor than its crude extract (50% inhibition) at the same concentrations (200 μg/mL).
Further comparisons can also be observed by the contrast in results obtained for TYR assays from the use of substrates L-DOPA and L-tyrosine. Here, several plant extracts have proven to be more effective in targeting the inhibition of the oxidation of either L-DOPA or L-tyrosine. This is illustrated by the TYR assay results obtained for Haloxylon articulatum, Greyia radlkoferi, Pituranthos scoparius, Myrsine africana, Hyaenanche globose, and Cleome Arabica [40,45,57]. Additional studies also included extracts of Dolichopentas longiflora, where preparations exhibited a stimulatory response on melanogenesis, whereas the IC50 value for TYR activity (26 ± μg/mL) showed contrasting results. This included Sesamum angolense of the same study, where pellets of the cells that were treated with the extract indicated no significant inhibition. However, the IC50 value (24 μg/mL) obtained indicated that the plant extract can illicit an inhibitory response [64]. Due to the complexity of pigment production, melanogenesis regulation takes place at different levels and various means of interference are possible—providing a possible explanation for the above-described contrasting results [85,86]. Mechanisms of depigmenting include; (1) tyrosinase inhibition, (2) decrease in DOPA polymerase, (3) induction of anti-inflammatory, and (4) anti-oxidant effects [87].
Extracts from the Lamiaceae family also proved to be effective inhibitors with Plectranthus ecklonii showing an IC50 value of 21.58 μg/mL with more than 70% TYR inhibition and Salvia officinalis decreasing MC to 27% at various concentrations [44,66,67]. In addition, other plant extracts elicited a significant inhibitory response on both melanin and TYR activities. These include Garcinia livingstonei and Garcinia kola (Clustiaceae), Myrsine africana (Myrsinaceae), Protea madiensis, and Serruria furcellata—both from the Proteaceae family and Sideroxylon inerme (Sapotaceae). Species from other families such as Clustiaceae (Garcinia livingstonei and Garcinia kola) exhibited significant activities with G. livingstonei exhibiting a large decrease of melanin concentration at 25 μg/mL and the seeds of G. kola inhibiting 79% of tyrosinase at 500 μg/mL [22,61].

4. Conclusions

Several studies have been conducted to identify inhibitors from both natural and synthetic sources, and a number of research papers have been published and regularly updated in this aspect. This study was conducted as a means of identifying plant-based skin lightening alternatives to the current toxic substances. Despite the serious and life-threatening complications associated with the chronic use of these products, the use of skin lighteners is still a widespread and common practice in several African countries [88,89].
All plants identified in this study showed competent antimelanogenesis and antityrosinase capabilities, with the most effective of the extracts being the following: Acacia nilotica, Cassipourea congoensis, Cassipourea flanaganii, Garcinia kola, Greyia flanaganii, Greyia radlkoferi, Hyaenanche globosa, Myrsine africana, Ormocarpum trichocarpum, Plectranthus ecklonii, Protea madiensis, Rorippa nasturtium-aquaticum, Serruria furcellata, Sesamum angolense, and Vachellia karroo. The reproducibility of the identified studies and interpretation of the results is limited by the inconsistencies in methodologies and means of plant extraction in these studies. Other variables also include geographical location and varied climate regions.
This review shows that plants of the African continent have the potential to act as melanin and TYR inhibitors and can be used to replace synthetic and other derived chemicals. Although many of these plants have been effective in their pigment reduction properties, plants are still known to cause allergic reactions and elicit phototoxic effects [87]. This is due to natural products being a complex mixture of chemical compounds, a fact often unknown to consumers. To combat this, extracts should be chemically characterized with respect to the product composition [30]. In addition, it is imperative that toxicity studies be conducted to establish a safe dose range. These findings could aid in the production and commercialization of these plants in natural-based remedies for cosmetic and skincare product industries.

Author Contributions

All authors listed made substantial contribution to the manuscript and qualify for authorship. No authors have been omitted. Concept and design: F.R.; data collection and interpretation of data: L.O., F.R., M.D.K., J.K.; writing—review and editing: L.O., F.R., M.D.K., J.K.; supervision: F.R.; project administration: L.O. All authors have read and agreed to the published version of the manuscript.

Funding

The authors greatly appreciate funding received by the National Research Foundation (NRF) South Africa for this study.

Acknowledgments

The authors would like to thank research assistants Jade Harris (JH) and Pedro Abrantes (PA) for assisting in the data collection.

Disclaimer

Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and, therefore, the NRF does not accept any liability in regard thereto.

Conflicts of Interest

The authors declare that they have no competing interests.

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Cosmetics 07 00060 sch001 550
Scheme 1. Illustration of the melanogenesis pathway.
Scheme 1. Illustration of the melanogenesis pathway.
Cosmetics 07 00060 sch001
Table
Table 1. Summary of the plant species identified and their melanin results.
Table 1. Summary of the plant species identified and their melanin results.
FamilyPlant NameRegionPart
Used		Results Reference
AnacardiaceaeHarpephyllum caffrumSA (EC)Leaves
Bark		26% melanin inhibition at 6.25 μg/mL[32]
ChenopodiaceaeArthrophytum scopariumTUNStems52% melanin inhibition [43]
ClusiaceaeGarcinia livingstoneiClusiaceaeBarkisolated compounds,
<0.25 MC at 25 μg/mL		[22]
LamiaceaeSalvia officinalisEGYAerial partsMC at 27% at 10, 20 and 40 μg/mL[44]
MelianthaceaeGreyia flanaganiiSA (EC)Leaves20% melanin inhibition at 6.25 μg/mL[4]
MelianthaceaeGreyia radlkoferiSA (MP)Leavesisolated compound,
50% melanin inhibition at 12.5 μg/mL		[45]
MyrsinaceaeMyrsine africanaSA
(EC, FS, GAU, KZN,
LP, MP, NW, WC)		Shoots50% melanin inhibition at 50 μg/mL[46]
MyrsinaceaeMyrsine africanaSA
(EC, FS, GAU, KZN,
LP, MP, NW, WC)		Shoots18% melanin inhibition at 12.50 μg/mL[40]
PedaliaceaeSesamum angolenseRWALeavescell pellets indicate no significant inhibition[47]
PicrodendraceaeHyaenanche globosaSA (WC)Leaves
Roots
Stems		-[40]
ProteaceaeProtea madiensisNIG, ETHRoot barkcell pellets indicates strong inhibition [47]
ProteaceaeSerruria furcellataSA (WC)Aerial parts94.3% melanin inhibition at 50 μg/mL[48]
RhizophoraceaCassipourea congoensisSEN, NIG, DRC, UGA, TZA, MLIRootsisolated compounds,
<0.2 pg/mL MC at 10 μg/mL and 100 μg/mL		[49]
RubiaceaeDolichopentas longifloraRWALeaves
Roots		cell pellet indicate increase [47]
SapotaceaeArgania spinosaMARFruits55% melanin inhibition at 50 μg/mL [50]
SapotaceaeArgania spinosaMARFruits>50% melanin inhibition at 1/100 [23]
SapotaceaeSideroxylon inermeSA (KZN)Stem-bark37% melanin inhibition at 6.2 μg/mL[33,51]
SapotaceaeVitellaria paradoxaMLI, ETH, UGAFruitCameroon = 10.1% MC at 100 μg/mL
Chad = 10.2% MC at 100 μg/mL
Sudan = 10.9% MC at 100 μg/mL 		[52]
ThymelaeaceaeThymelaea hirsutaTUNLeaves> 50% melanin inhibition of melanin[53]
ThymelaeaceaeThymelaea hirsutaTUNLeavesisolated compound,
37% melanin inhibition at 0.1 μg/mL		[54]
ThymelaeaceaeThymelaea hirsutaTUNLeavesisolated compound,
50% melanin inhibition at 1 μg/mL
isolated compound,
33% melanin inhibitionat 0.1 μg/mL		[55]
This table indicates the melanin inhibition or MC (melanin content) at various concentrations of plant extracts (µg/mL). Provinces in South Africa (SA)—EC: Eastern Cape; FS: Free State; GAU: Gauteng; KZN: KwaZulu-Natal; LP: Limpopo; MP: Mpumalanga; NW: North West; WC: Western Cape; Other African countries—ALG: Algeria; DRC: Democratic Republic of Congo; EGY: Egypt; ETH: Ethiopia; GHA: Ghana; IC: Ivory Coast; MAR: Morocco; MLI: Malawi; NIG: Nigeria; RWA: Rwanda; SEN: Senegal; SUD: Sudan; TUN: Tunisia; TZA: Tanzania; UGA: Uganda; -: not significant.
Table
Table 2. Summary of the plant species identified and their tyrosinase results.
Table 2. Summary of the plant species identified and their tyrosinase results.
Family Plant NameRegionPart
Used		Results (IC50 or Other Values)Reference
AnacardiaceaeHarpephyllum caffrumSA (EC)Leaves
Bark		92% inhibition of L-tyrosine at 500 μg/mL
60% inhibition of L-DOPA at 500 μg/mL
IC50 40 ± 0.035 μg/mL		[32]
AnacardiaceaeHyaenanche globosaSA (EC)Leaves
Bark		L-DOPA at 500 μg/mL = 42% inhibition
L-tyrosine at 500 μg/mL = 92% inhibition
IC50 27.1 ± 042 μg/mL		[40]
AnacardiaceaeHyaenanche globosaSA (EC)Leaves
Bark		90.4% TYR inhibition at 200 μg/mL[56]
ApiaceaePituranthos scopariusTUNAerial partsIC50 125.01 ± 0.72 μg/mL using L-tyrosine
IC50 270.51 ± 0.76 μg/mL using L-DOPA		[57]
AsteraceaeHelichrysum niveumSA (WC)Aerial partsIsolated compound, 35.63 ± 4.67 μg/mL[58]
BrassiaceaeRorippa nasturtium-aquaticumSA (EC)LeavesIC50 22.24 μg/mL[59]
BrassiaceaeRorippa nasturtium-aquaticumSA (EC)LeavesIC50 1.513 μg/mL[60]
CapparaceaeCleome arabicaTUNAerial partsIC50 124.4 ± 0.69 μg/mL L-tyrosine
IC50 243.43 ± 2.71 μg/mL using L-DOPA		[57]
ChenopodiaceaeHaloxylon articulatumALG, MAR, TUNShootIC50 160 μg/mL using L-DOPA as substrate
IC50 using L- tyrosine not significant		[57]
ClusiaceaeGarcinia kolaALGSeed79% TYR inhibition at 500 μg/mL[61]
EuphorbiaceaeMacaranga hurifoliaNIG, GHALeaves
Stem bark		Leaf extracts = 159.42 mg KAE/g
Bark extracts = 160.42 mg KAE/g		[62]
FabaceaeCeratonia siliquaALGLeavescrude extract, 50% TYR inhibition at 200 μg/mL
isolate compounds, 90% TYR inhibition at 200 μg/mL		[51]
FabaceaeOrmocarpum trichocarpumSA (KZN, LP, MP)Leaves
Stems		IC50 2.95 ± 1.76 μg/mL using L-tyrosine[63]
FabaceaeRhynchosia villosaSA (EC, KZN, MP)Root56.40% TYR inhibition at 100 μg/mL[64]
FabaceaeVachellia karrooSA
(EC, FS, GAU, KZN,
MP, NC, NW, WC)		RootsIC50 6.84 μg/mL[63]
FabaceaeAcacia niloticaSUD
SA (GAU, KZN, LP, MP, NW)		Pods
Bark		pod extract,
IC50 8.61 ± 0.94 μg/mL using L-tyrosine
pod extract,
98.3% TYR inhibition at 500 μg/mL 		[36]
FabaceaeAcacia niloticaSUD
SA (GAU, KZN, LP, MP, NW)		Pods
Bark		IC50 12.97 ± 1.07 μg/mL[65]
LamiaceaePlectranthus eckloniiSA (EC, KZN, MP)Aerial partsIC50 61.73 ± 2.69 μg/mL
>70% at 100 μg/mL		[66]
LamiaceaePlectranthus eckloniiSA (EC, KZN, MP)Aerial partsIC50 21.58 μg/mL[67]
LamiaceaeSalvia barrelieriALGAerial parts27% TYR inhibition at 1.5 mg/mL[68]
MelianthaceaeGreyia flanaganiiSA (EC)Leaves95% TYR inhibition at 200 μg/mL
Isolated compound, IC50 17.86 μg/mL		[4]
MelianthaceaeBersama abyssinicaICLeaves148.94 mg KAE/g[69]
MelianthaceaeGreyia radlkoferiSA (MP)LeavesIC50 17.96 μg/mL using L-tyrosine
IC50 using L- DOPA not significant		[45]
MyrsinaceaeMyrsine africanaSA
(EC, FS, GAU, KZN,
LP, MP, NW, WC)		ShootsIC50 0.12 ± 0.001 mg/mL[46]
MyrsinaceaeMyrsine africanaSA
(EC, FS, GAU, KZN,
LP, MP, NW, WC)		ShootsL-DOPA at 500 μg/mL = 62% inhibition
L-tyrosine at 500 μg/mL = 83% inhibition
IC50 22.51 ± 0.42 μg/mL		[40]
MyrsinaceaeMyrsine africanaSA
(EC, FS, GAU, KZN,
LP, MP, NW, WC)		ShootsIC50 27.4 μg/mL using L-tyrosine[63]
PedaliaceaeSesamum angolenseRWALeavesIC50 24 μg/mL[47]
PoaceaeSorghum bicolorTUNStalk40% TYR inhibition (in comparison to untreated control)[70]
PodocarpaceaePodocarpus elongatesSA (KZN)Stems74% TYR inhibition at 1 mg/mL
EC50 = 0.14mg/mL		[35]
ProteaceaeProtea madiensisNIG, ETHRoot bark
Leaves		31 ± 4 μg/mL[47]
ProteaceaeSerruria furcellataSA (WC)Aerial parts95.49% TYR inhibition at 200 μg/mL
80.84% TYR inhibition at 50 μg/mL		[48]
RhizophoraceaeCassipourea congoensisSEN, NIG, DRC, UGA, TZA, MLIRootscrude extract,
>80% TYR inhibition at 10 μg/mL and 100 μg/mL		[49]
RhizophoraceaeCassipourea flanaganiiSA (EC, KZN)BarkIC50 22.24 ± 1.32 μg/mL[59]
RhizophoraceaeCassipourea flanaganiiSA (EC, KZN)BarkIC50 1.425 μg/mL[60]
RubiaceaeDolichopentas longifloraRWALeaves
Roots		IC50 26 ± 2 μg/mL[47]
SapotaceaeSideroxylon inermeSA (KZN)Stem-bark70% TYR inhibition at 200 μg/mL[33,51]
This table indicates TYR inhibition, EC50 (concentration at which the plant extract exhibits 50% of its maximum response) and IC50 (concentration at which half the original TYR activity is inhibited) values of plant extracts (µg/mL or KAE/g; KA equivalent per grams or mg/mL). Provinces in South Africa (SA) - EC: Eastern Cape; FS: Free State; GAU: Gauteng; KZN: KwaZulu-Natal; LP: Limpopo; MP: Mpumalanga; NW: North West; WC: Western Cape; Other African countries - ALG: Algeria; DRC: Democratic Republic of Congo; EGY: Egypt; ETH: Ethiopia; GHA: Ghana; IC: Ivory Coast; MAR: Morocco; MLI: Malawi; NIG: Nigeria; RWA: Rwanda; SEN: Senegal; SUD: Sudan; TUN: Tunisia; TZA: Tanzania; UGA: Uganda.

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Opperman, L.; De Kock, M.; Klaasen, J.; Rahiman, F. Tyrosinase and Melanogenesis Inhibition by Indigenous African Plants: A Review. Cosmetics 2020, 7, 60. https://doi.org/10.3390/cosmetics7030060

AMA Style

Opperman L, De Kock M, Klaasen J, Rahiman F. Tyrosinase and Melanogenesis Inhibition by Indigenous African Plants: A Review. Cosmetics. 2020; 7(3):60. https://doi.org/10.3390/cosmetics7030060

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Opperman, Laurentia, Maryna De Kock, Jeremy Klaasen, and Farzana Rahiman. 2020. "Tyrosinase and Melanogenesis Inhibition by Indigenous African Plants: A Review" Cosmetics 7, no. 3: 60. https://doi.org/10.3390/cosmetics7030060

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Opperman, L., De Kock, M., Klaasen, J., & Rahiman, F. (2020). Tyrosinase and Melanogenesis Inhibition by Indigenous African Plants: A Review. Cosmetics, 7(3), 60. https://doi.org/10.3390/cosmetics7030060

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