Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben
1
Bionics Program, Tokyo University of Technology Graduate School, Tokyo 192-0982, Japan
2
School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 192-0982, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Perry Xiao
Cosmetics 2017, 4(4), 38; https://doi.org/10.3390/cosmetics4040038
Received: 29 July 2017
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Revised: 4 September 2017
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Accepted: 21 September 2017
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Published: 25 September 2017
This study investigated the effect of amino carbonylation (Maillard reaction) on the function of the epidermal basement membrane (BM) by analyzing epidermal cell proliferation and keratinization and stratum corneum barrier function using a three-dimensional human epidermal BM model treated with glyceraldehyde. Intracellular ATP levels were lower in cells cultured on amino-carbonylated epidermal BM as compared to those in normal epidermal BM (control). Moreover, trans-epidermal water loss was increased by culturing on amino-carbonylated BM relative to the control; this was accompanied by downregulation of filaggrin, transglutaminase-1, and serine palmitoyltransferase 2 mRNA levels. p-Hydroxybenzoic acid methyl ester (methylparaben) abrogated the decrease in ATP production and filaggrin expression in human keratinocytes induced by amino-carbonylated collagen. Thus, amino carbonylation of the epidermal BM inhibits moisture retention, keratinization, and ceramide synthesis and disrupts the barrier function of the stratum corneum. These findings suggest that methylparaben can be an effective additive to cosmetics for improving epidermal function that is compromised by amino carbonylation.
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Keywords:
advanced glycation end products; amino carbonylation; epidermal basement membrane; filaggrin; transglutaminase; ceramide
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MDPI and ACS Style
Morimoto, H.; Gu, L.; Zeng, H.; Maeda, K. Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben. Cosmetics 2017, 4, 38. https://doi.org/10.3390/cosmetics4040038
AMA Style
Morimoto H, Gu L, Zeng H, Maeda K. Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben. Cosmetics. 2017; 4(4):38. https://doi.org/10.3390/cosmetics4040038
Chicago/Turabian StyleMorimoto, Haruka, Lihao Gu, Haifeng Zeng, and Kazuhisa Maeda. 2017. "Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben" Cosmetics 4, no. 4: 38. https://doi.org/10.3390/cosmetics4040038
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