Next Article in Journal
Cosmetic Potential of Marine Fish Skin Collagen
Previous Article in Journal
Improving Skin Hydration and Age-related Symptoms by Oral Administration of Wheat Glucosylceramides and Digalactosyl Diglycerides: A Human Clinical Study
Open AccessArticle

Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben

1
Bionics Program, Tokyo University of Technology Graduate School, Tokyo 192-0982, Japan
2
School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 192-0982, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Perry Xiao
Cosmetics 2017, 4(4), 38; https://doi.org/10.3390/cosmetics4040038
Received: 29 July 2017 / Revised: 4 September 2017 / Accepted: 21 September 2017 / Published: 25 September 2017
This study investigated the effect of amino carbonylation (Maillard reaction) on the function of the epidermal basement membrane (BM) by analyzing epidermal cell proliferation and keratinization and stratum corneum barrier function using a three-dimensional human epidermal BM model treated with glyceraldehyde. Intracellular ATP levels were lower in cells cultured on amino-carbonylated epidermal BM as compared to those in normal epidermal BM (control). Moreover, trans-epidermal water loss was increased by culturing on amino-carbonylated BM relative to the control; this was accompanied by downregulation of filaggrin, transglutaminase-1, and serine palmitoyltransferase 2 mRNA levels. p-Hydroxybenzoic acid methyl ester (methylparaben) abrogated the decrease in ATP production and filaggrin expression in human keratinocytes induced by amino-carbonylated collagen. Thus, amino carbonylation of the epidermal BM inhibits moisture retention, keratinization, and ceramide synthesis and disrupts the barrier function of the stratum corneum. These findings suggest that methylparaben can be an effective additive to cosmetics for improving epidermal function that is compromised by amino carbonylation. View Full-Text
Keywords: advanced glycation end products; amino carbonylation; epidermal basement membrane; filaggrin; transglutaminase; ceramide advanced glycation end products; amino carbonylation; epidermal basement membrane; filaggrin; transglutaminase; ceramide
Show Figures

Figure 1

MDPI and ACS Style

Morimoto, H.; Gu, L.; Zeng, H.; Maeda, K. Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben. Cosmetics 2017, 4, 38.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop