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Article

Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User

1
Department of Therapeutic Research and Medicines Evaluation, National Institute of Health, V.le Regina Elena 299, 00161 Rome, Italy
2
Centro Nazionale Organismo Notificato Dispositivi e Cosmetici (ONDICO), National Institute of Health, V.le Regina Elena 299, 00161 Rome, Italy
*
Author to whom correspondence should be addressed.
Cosmetics 2014, 1(2), 82-93; https://doi.org/10.3390/cosmetics1020082
Received: 31 January 2014 / Revised: 31 March 2014 / Accepted: 6 April 2014 / Published: 15 April 2014
(This article belongs to the Special Issue Analytical Methods for Quality Control of Cosmetics)
A fast and sensitive ultra-performance liquid chromatography tandem mass spectrometry method was developed for the measurement of caffeine in caffeine-laced pants and in urine and skin of a pants user. The substance and its internal standard (N-ethylnorcotinine) were separated by reversed phase chromatography with 5 mM ammonium formate pH 3.0 and 0.3% formic acid in acetonitrile mobile phase (83:17 v/v) by isocratic elution and detected by tandem mass spectrometry operated in multiple reaction monitoring mode via positive electrospray ionization. Linearity was studied from 1.4 to100 ng/mL range for urine, from 5 to 100 ng/cotton swab for skin caffeine and from 1.3 to 100 µg/samples for 4 cm2 textile samples. Good determination coefficients (r2 = 0.99) were found in all cases. At three concentrations spanning the linear dynamic ranges of different samples mean recoveries of caffeine were always higher than 80% and intra-assay and inter-assay imprecision and inaccuracy were always better than 105%. For the first time, caffeine content in this cosmetotextile was determined together with the measurement of caffeine released on the user skin, the absorbed amount with resulting urinary concentrations. View Full-Text
Keywords: cosmetotextile; caffeine laced pants; ultra-performance liquid chromatography; tandem mass spectrometry cosmetotextile; caffeine laced pants; ultra-performance liquid chromatography; tandem mass spectrometry
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MDPI and ACS Style

Pellegrini, M.; De Orsi, D.; Guarino, C.; Rotolo, M.C.; Di Giovannandrea, R.; Pacifici, R.; Pichini, S. Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User. Cosmetics 2014, 1, 82-93. https://doi.org/10.3390/cosmetics1020082

AMA Style

Pellegrini M, De Orsi D, Guarino C, Rotolo MC, Di Giovannandrea R, Pacifici R, Pichini S. Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User. Cosmetics. 2014; 1(2):82-93. https://doi.org/10.3390/cosmetics1020082

Chicago/Turabian Style

Pellegrini, Manuela, Daniela De Orsi, Carmine Guarino, Maria C. Rotolo, Rita Di Giovannandrea, Roberta Pacifici, and Simona Pichini. 2014. "Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User" Cosmetics 1, no. 2: 82-93. https://doi.org/10.3390/cosmetics1020082

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