Next Article in Journal
Evaluation of Inflammation Caused by Cardiopulmonary Bypass in a Small Animal Model
Previous Article in Journal
Matrix Metalloproteases in Pancreatic Ductal Adenocarcinoma: Key Drivers of Disease Progression?
Open AccessArticle

Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon

Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø - The Artic University of Norway, N-9037 Tromsø, Norway
Cermaq Ltd., Gjærbakknes, N-8286 Steigen, Norway
Val FoU AS, Val, N-7970 Kolvereid, Norway
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
NOFIMA, Muninbakken 9-13, N-9291 Tromsø, Norway
Authors to whom correspondence should be addressed.
Equal contribution.
Biology 2020, 9(4), 82;
Received: 25 March 2020 / Revised: 13 April 2020 / Accepted: 15 April 2020 / Published: 20 April 2020
(This article belongs to the Section Immunology)
The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-γ, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ß genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-γ, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ß in the muscles of fish injected with TGF-ß-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ß on both bacterial infections were negligible. View Full-Text
Keywords: overexpression; T-bet; GATA-3; TGF-ß; Atlantic salmon; gene expression overexpression; T-bet; GATA-3; TGF-ß; Atlantic salmon; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Slettjord, T.H.; Sekkenes, H.J.; Chi, H.; Bøgwald, J.; Swain, T.; Dalmo, R.A.; Kumari Swain, J. Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon. Biology 2020, 9, 82.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop