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Open AccessArticle

Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon

1
Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø - The Artic University of Norway, N-9037 Tromsø, Norway
2
Cermaq Ltd., Gjærbakknes, N-8286 Steigen, Norway
3
Val FoU AS, Val, N-7970 Kolvereid, Norway
4
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
5
NOFIMA, Muninbakken 9-13, N-9291 Tromsø, Norway
*
Authors to whom correspondence should be addressed.
Equal contribution.
Biology 2020, 9(4), 82; https://doi.org/10.3390/biology9040082
Received: 25 March 2020 / Revised: 13 April 2020 / Accepted: 15 April 2020 / Published: 20 April 2020
(This article belongs to the Section Immunology)
The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-γ, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ß genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-γ, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ß in the muscles of fish injected with TGF-ß-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ß on both bacterial infections were negligible. View Full-Text
Keywords: overexpression; T-bet; GATA-3; TGF-ß; Atlantic salmon; gene expression overexpression; T-bet; GATA-3; TGF-ß; Atlantic salmon; gene expression
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MDPI and ACS Style

Slettjord, T.H.; Sekkenes, H.J.; Chi, H.; Bøgwald, J.; Swain, T.; Dalmo, R.A.; Kumari Swain, J. Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon. Biology 2020, 9, 82.

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