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Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation

Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania
Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095 Bucharest, Romania
National Institute for Lasers, Plasma and Radiation Physics, Atomistilor 409, 077125 Magurele, Bucharest, Romania
Faculty of Mathematics and Natural Sciences, University of Craiova, 200585 Craiova, Romania
Faculty of Physics, University of Bucharest, RO-077125 Magurele, Bucharest, Romania
School of Engineering and Design, Brunel University, London UB8 3PH, UK
Authors to whom correspondence should be addressed.
Coatings 2019, 9(4), 236;
Received: 28 February 2019 / Revised: 25 March 2019 / Accepted: 2 April 2019 / Published: 4 April 2019
(This article belongs to the Special Issue Current Research in Pulsed Laser Deposition)
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The improvement in the research area of the implant by surface functionalization when correlated with the biological response is of major interest in the biomedical field. Based on the fact that the inflammatory response is directly involved in the ultimate response of the implant within the body, it is essential to study the macrophage-material interactions. Within this context, we have investigated the composite material-macrophage cell interactions and the inflammatory response to these composites with amorphous hydroxyapatite (HA), Lactoferrin (Lf), and polyethylene glycol-polycaprolactone (PEG-PCL) copolymer. All materials are obtained by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique and characterized by Atomic Force Microscopy and Scanning Electron Microscopy. Macrophage-differentiated THP-1 cells proliferation and metabolic activity were assessed by qualitative and quantitative methods. The secretion of tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) cytokine, in the presence and absence of the inflammatory stimuli (bacterial endotoxin; lipopolysaccharide (LPS)), was measured using an ELISA assay. Our results revealed that the cellular response depended on the physical-chemical characteristics of the coatings. Copolymer-HA-Lf coatings led to low level of pro-inflammatory TNF-α, the increased level of anti-inflammatory IL-10, and the polarization of THP-1 cells towards an M2 pro-reparative phenotype in the presence of LPS. These findings could have important potential for the development of composite coatings in implant applications. View Full-Text
Keywords: composite coatings; MAPLE; Lactoferrin; macrophage interactions composite coatings; MAPLE; Lactoferrin; macrophage interactions

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Icriverzi, M.; Rusen, L.; Brajnicov, S.; Bonciu, A.; Dinescu, M.; Cimpean, A.; Evans, R.W.; Dinca, V.; Roseanu, A. Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation. Coatings 2019, 9, 236.

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