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Article

Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant

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IRCCS Istituto Ortopedico Rizzoli, Complex Structure of Surgical Sciences and Technologies, Via di Barbiano 1/10, 40136 Bologna, Italy
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IRCCS Istituto Ortopedico Rizzoli, Complex Structure of Reconstructive Orthopedic Surgery Innovative Techniques, Via G.C. Pupilli 1, 40136 Bologna, Italy
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Author to whom correspondence should be addressed.
Academic Editor: Eugenio Velasco-Ortega
Coatings 2021, 11(7), 840; https://doi.org/10.3390/coatings11070840
Received: 18 June 2021 / Revised: 8 July 2021 / Accepted: 10 July 2021 / Published: 12 July 2021
(This article belongs to the Special Issue Bioactive Coatings on Medical Implants)
Bone regeneration remains one of the major clinical needs in orthopedics, and advanced and alternative strategies involving bone substitutes, cells, and growth factors (GFs) are mandatory. The purpose of this study was to evaluate whether the association of autologous bone marrow mesenchymal stem cells (BMSC), isolated by ‘one-step surgical procedure’, and activated platelet rich plasma (PRP) improves osseointegration and bone formation of a hydroxyapatite-coated titanium (Ti-HA) implant, already in clinical use, in a rabbit cancellous defect. The GFs present in plasma, in inactivated and activated PRP were also tested. At 2 weeks, histology and histomorphometry highlighted increased bone-to-implant contact (BIC) in Ti-HA combined with BMSC and PRP in comparison to Ti-HA alone and Ti-HA + PRP. The combined effect of BMSC and PRP peaked at 4 weeks where the BIC value was higher than all other treatments. At both experimental times, newly formed bone (Trabecular Bone Volume, BV/TV) in all tested treatments showed increased values in comparison to Ti-HA alone. At 4 weeks Ti-HA + PRP + BMSC showed the highest BV/TV and the highest osteoblasts number; additionally, a higher osteoid surface and bone formation rate were found in Ti-HA + BMSC + PRP than in all other treatments. Finally, the analyses of GFs revealed higher values in the activated PRP in comparison to plasma and to non-activated PRP. The study suggests that the combination of autologous activated PRP, as a carrier for BMSCs, is a promising regenerative strategy for bone formation, osseointegration, and mineralization of bone implants. View Full-Text
Keywords: osseointegration; PRP; BMSCs; bone regeneration; hydroxyapatite; titanium; implant osseointegration; PRP; BMSCs; bone regeneration; hydroxyapatite; titanium; implant
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MDPI and ACS Style

Salamanna, F.; Del Piccolo, N.; Sartori, M.; Giavaresi, G.; Martini, L.; Di Sante, G.; Stagni, C.; Dallari, D.; Fini, M. Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant. Coatings 2021, 11, 840. https://doi.org/10.3390/coatings11070840

AMA Style

Salamanna F, Del Piccolo N, Sartori M, Giavaresi G, Martini L, Di Sante G, Stagni C, Dallari D, Fini M. Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant. Coatings. 2021; 11(7):840. https://doi.org/10.3390/coatings11070840

Chicago/Turabian Style

Salamanna, Francesca, Nicolandrea Del Piccolo, Maria Sartori, Gianluca Giavaresi, Lucia Martini, Giuseppe Di Sante, Cesare Stagni, Dante Dallari, and Milena Fini. 2021. "Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant" Coatings 11, no. 7: 840. https://doi.org/10.3390/coatings11070840

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