Next Article in Journal
Effects of Inorganic ZnO Particle Doping on Crystalline Polymer Morphology and Space Charge Behavior
Previous Article in Journal
Additional Tribological Effect of Laser Surface Texturing and Diamond-Like Carbon Coating for Medium Carbon Steel at Near Room Temperature
Previous Article in Special Issue
Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings
Due to scheduled maintenance work on our core network, there may be short service disruptions on this website between 16:00 and 16:30 CEST on September 25th.
Article

Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques

1
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via A. Moro 2, 53100 Siena, Italy
2
Procter & Gamble Ltd.—Newcastle Innovation Centre, Whitley Road, Longbenton, Newcastle upon Tyne NE12 9BZ, UK
*
Authors to whom correspondence should be addressed.
Both authors contributed equally to this work.
Coatings 2020, 10(10), 930; https://doi.org/10.3390/coatings10100930
Received: 4 September 2020 / Revised: 24 September 2020 / Accepted: 25 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Antibacterial Coatings and Biofilm)
In the present study, the early stage of bacteria biofilm formation has been studied as a function of different nutrients. Infrared spectra of Pseudomonas fluorescens (PF) and Staphylococcus epidermidis (SE), on germanium ATR crystal, were collected under deionized water H2O, phosphate buffered solution (PBS) and PBS with glucose (PBS-G). In H2O, protein bands of PF increased while, no difference in PBS and PBS-G were observed until 135 min. SE strain showed a low sensitivity to PBS composition starting to expose proteins on surfaces after 120 min. SE shows a low polysaccharides increase in H2O while, in bare and enriched PBS their intensity increases after 120 and 75 min. in PBS and PBS-G respectively. PF exhibits a peculiar behavior in H2O where the saccharide bands increased strongly after 100 min, while under all the other conditions, the intensity of polysaccharide bands increased up to the plateau probably because the layer of the biofilm exceeded the penetration capability of FTIR technique. All data suggest that, under lack of nutrients, both the bacteria tend to firmly anchor themselves to the support using proteins. View Full-Text
Keywords: Fourier transform infrared spectroscopy; attenuated total reflectance; bacteria; in-situ analysis; ATR-FTIR; vibrational spectroscopy; 2nd order derivative method Fourier transform infrared spectroscopy; attenuated total reflectance; bacteria; in-situ analysis; ATR-FTIR; vibrational spectroscopy; 2nd order derivative method
Show Figures

Figure 1

MDPI and ACS Style

Consumi, M.; Jankowska, K.; Leone, G.; Rossi, C.; Pardini, A.; Robles, E.; Wright, K.; Brooker, A.; Magnani, A. Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques. Coatings 2020, 10, 930. https://doi.org/10.3390/coatings10100930

AMA Style

Consumi M, Jankowska K, Leone G, Rossi C, Pardini A, Robles E, Wright K, Brooker A, Magnani A. Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques. Coatings. 2020; 10(10):930. https://doi.org/10.3390/coatings10100930

Chicago/Turabian Style

Consumi, Marco, Kamila Jankowska, Gemma Leone, Claudio Rossi, Alessio Pardini, Eric Robles, Kevin Wright, Anju Brooker, and Agnese Magnani. 2020. "Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques" Coatings 10, no. 10: 930. https://doi.org/10.3390/coatings10100930

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop