Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine
Abstract
:1. Introduction
2. Results
2.1. Core Genome-Based Confirmation on Species Level and Clustering with International Outbreak Strains
2.2. Identified Molecular Resistance Mechanisms
3. Discussion
4. Materials and Methods
4.1. Patient Isolates
4.2. DNA Extraction and Whole Genome Sequencing
4.3. Molecular Epidemiology and Determination of Antibiotic Resistance Genes
4.4. Ethical Clearance
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample (rep-PCR Cluster) | MLST | Clonal Lineage | Antibiotic Resistance Determinants | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
STox | STpas | Sulphonamide | Phenicol | Beta-Lactam | Aminoglycoside | Macrolide | Tetracycline | Trimethoprim | Fluoroquinolone and Aminoglycoside | ||
V86041-BER-HH (rep-PCR 1) | 1102 | 2 | IC2 | blaADC-11, blaOXA-23, blaOXA-66 | aac(3)-Ia-like, aadA1-like, aph(3′)-Ic, aph(3′)-VIa-like | ||||||
V86039-BER-HH (rep-PCR 2) | 231 | 1 | IC1 | sul1 | catA1-like, cmlA1-like | blaADC-158, blaGES-12, blaOXA-23, blaOXA-69 | aac(3)-Ia-like, aadA1, aadA2, aadB, aph(3′)-VIa-like, strA-like, strB-like | tet(A) | dfrA7 | aac(6′)Ib-cr-like | |
V66728-3-KOB (rep-PCR 3) | 231 | 1 | IC1 | sul1 | cmlA1-like | blaADC-158, blaGES-12, blaOXA-69 | aadA2, aadB, aph(3′)-VIa, strA-like, strB-like | dfrA7 | aac(6′)Ib-cr-like | ||
V77717-2-KOB (rep-PCR 3 outlier) | 440 | 25 | IC7 | sul2 | blaADC-26-like, blaOXA-64 | aadB-like, aph(3′)-Ic, strA, strB-like | |||||
V60248_1_KOB (rep-PCR 4) | 944 | 78 | IC6 | sul1 | blaADC-152, blaOXA-90, blaOXA-72 | aadA5, armA | mph(E) | ||||
V86042_BER_HH (rep-PCR 4 outlier) | 944 | 78 | IC6 | sul1, sul2-like | catA1-like, floR-like | blaADC-152, blaCARB-16, blaCTX-M-115, blaOXA-90, blaOXA-72 | aadA5, armA | mph(E) | |||
V58143_5_KOB (singleton) | 2144 | 78 | IC6 | sul2-like | catA1-like, floR-like | blaADC-107, blaCTX-M-115, blaOXA-90, blaOXA-72 | |||||
V86030_BER_HH (singleton) | 690 | 25 | IC7 | sul2 | blaADC-26-like*, blaOXA-23, blaOXA-64 | aph(3′)-Ic, aph(3′)-VIa-like, strA-like, strB |
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Higgins, P.G.; Hagen, R.M.; Podbielski, A.; Frickmann, H.; Warnke, P. Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine. Antibiotics 2020, 9, 579. https://doi.org/10.3390/antibiotics9090579
Higgins PG, Hagen RM, Podbielski A, Frickmann H, Warnke P. Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine. Antibiotics. 2020; 9(9):579. https://doi.org/10.3390/antibiotics9090579
Chicago/Turabian StyleHiggins, Paul G., Ralf Matthias Hagen, Andreas Podbielski, Hagen Frickmann, and Philipp Warnke. 2020. "Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine" Antibiotics 9, no. 9: 579. https://doi.org/10.3390/antibiotics9090579