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Article

A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi-Infected Human Cells

1
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, Brazil
2
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, Brazil
3
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife 52171-900, Brazil
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(8), 515; https://doi.org/10.3390/antibiotics9080515
Received: 15 July 2020 / Revised: 7 August 2020 / Accepted: 12 August 2020 / Published: 14 August 2020
(This article belongs to the Special Issue Natural Compounds as Antimicrobial Agents, 2nd Volume)
Trypanosoma cruzi causes the lethal Chagas disease, which is endemic in Latin America. Flowers of Moringa oleifera (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to T. cruzi trypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by T. cruzi-infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LC50 of 43.5 µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index > 11.48 was determined. When T. cruzi-infected PBMCs were treated with MoFTI (43.5 or 87.0 µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity to T. cruzi and the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease. View Full-Text
Keywords: cytokines; cytotoxicity; immunomodulatory agent; Moringa oleifera; protease inhibitor; trypanocidal agent cytokines; cytotoxicity; immunomodulatory agent; Moringa oleifera; protease inhibitor; trypanocidal agent
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MDPI and ACS Style

Nova, I.C.V.; Moreira, L.R.; Torres, D.J.L.; Oliveira, K.K.d.S.; Patriota, L.L.d.S.; Coelho, L.C.B.B.; Paiva, P.M.G.; Napoleão, T.H.; Lorena, V.M.B.d.; Pontual, E.V. A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi-Infected Human Cells. Antibiotics 2020, 9, 515. https://doi.org/10.3390/antibiotics9080515

AMA Style

Nova ICV, Moreira LR, Torres DJL, Oliveira KKdS, Patriota LLdS, Coelho LCBB, Paiva PMG, Napoleão TH, Lorena VMBd, Pontual EV. A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi-Infected Human Cells. Antibiotics. 2020; 9(8):515. https://doi.org/10.3390/antibiotics9080515

Chicago/Turabian Style

Nova, Isabella C.V., Leyllane R. Moreira, Diego J.L. Torres, Kamila K.d.S. Oliveira, Leydianne L.d.S. Patriota, Luana C.B.B. Coelho, Patrícia M.G. Paiva, Thiago H. Napoleão, Virgínia M.B.d. Lorena, and Emmanuel V. Pontual 2020. "A Trypsin Inhibitor from Moringa oleifera Flowers Modulates the Immune Response In Vitro of Trypanosoma cruzi-Infected Human Cells" Antibiotics 9, no. 8: 515. https://doi.org/10.3390/antibiotics9080515

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