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Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba)

1
Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic
2
Institute of Biological Sciences, Campus Talca, Universidad de Talca, Talca 3465548, Chile
3
Agronomy School, Faculty of Agronomic and Food Sciences, Pontificia Universidad Católica de Valparaíso, Quillota 2260000, Chile
4
Regional Center for Studies in Healthy Food (CREAS), CONICYT-Regional GORE Valparaíso Project R17A10001, Avenida Universidad, Valparaíso 2340000, Chile
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(8), 444; https://doi.org/10.3390/antibiotics9080444
Received: 22 June 2020 / Revised: 13 July 2020 / Accepted: 21 July 2020 / Published: 25 July 2020
(This article belongs to the Special Issue A Selection of Studies Presented at Biotech 2020 Symposium)
Arrayan and peumo fruits are commonly used in the traditional medicine of Chile. In this study, the concentration of the extracts halving the bacterial viability and biofilms formation and disruption of the drug-sensitive and drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa was determined. The chemical composition of extracts was analyzed by high-resolution liquid chromatography coupled with mass spectrometry (U-HPLC/MS). The arrayan extract (Inhibitory concentration IC50 0.35 ± 0.01 mg/mL) was more effective than peumo extract (IC50 0.53 ± 0.02 mg/mL) in the inhibition of S. aureus planktonic cells. Similarly, the arrayan extract was more effective in inhibiting the adhesion (S. aureus IC50 0.23 ± 0.02 mg/mL, P. aeruginosa IC50 0.29 ± 0.02 mg/mL) than peumo extracts (S. aureus IC50 0.47 ± 0.03 mg/mL, P. aeruginosa IC50 0.35 ± 0.01 mg/mL). Both extracts inhibited quorum sensing in a concentration-dependent manner, and the most significant was the autoinducer-2 type communication inhibition by arrayan extract. Both extracts also disrupted preformed biofilm of P. aeruginosa (arrayan IC50 0.56 ± 0.04 mg/mL, peumo IC50 0.59 ± 0.04 mg/mL). However, neither arrayan nor peumo extracts disrupted S. aureus mature biofilm. U-HPLC/MS showed that both fruit extracts mainly possessed quercetin compounds; the peumo fruit extract also contained phenolic acids and phenylpropanoids. Our results suggested that both extracts could be used as natural antimicrobials for some skin and nosocomial infections. View Full-Text
Keywords: traditional medicine; drug-resistant bacteria; high-resolution HPLC/MS; biofilm disruption; quorum sensing traditional medicine; drug-resistant bacteria; high-resolution HPLC/MS; biofilm disruption; quorum sensing
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MDPI and ACS Style

Viktorová, J.; Kumar, R.; Řehořová, K.; Hoang, L.; Ruml, T.; Figueroa, C.R.; Valdenegro, M.; Fuentes, L. Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba). Antibiotics 2020, 9, 444. https://doi.org/10.3390/antibiotics9080444

AMA Style

Viktorová J, Kumar R, Řehořová K, Hoang L, Ruml T, Figueroa CR, Valdenegro M, Fuentes L. Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba). Antibiotics. 2020; 9(8):444. https://doi.org/10.3390/antibiotics9080444

Chicago/Turabian Style

Viktorová, Jitka; Kumar, Rohitesh; Řehořová, Kateřina; Hoang, Lan; Ruml, Tomas; Figueroa, Carlos R.; Valdenegro, Monika; Fuentes, Lida. 2020. "Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba)" Antibiotics 9, no. 8: 444. https://doi.org/10.3390/antibiotics9080444

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