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Simple and Accurate HPTLC-Densitometric Method for Quantification of Delafloxacin (A Novel Fluoroquinolone Antibiotic) in Plasma Samples: Application to Pharmacokinetic Study in Rats
Open AccessArticle

Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

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Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
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Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
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Drug Bioavailability Unit, Central Laboratory, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
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Micro-Analytical Laboratory, Applied Organic Chemistry Department, National Research Center, Dokki, Cairo 12622, Egypt
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Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
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Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
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Author to whom correspondence should be addressed.
Antibiotics 2020, 9(6), 359; https://doi.org/10.3390/antibiotics9060359
Received: 2 June 2020 / Revised: 18 June 2020 / Accepted: 22 June 2020 / Published: 25 June 2020
(This article belongs to the Special Issue Challenges in Antibiotics Analysis)
In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX. View Full-Text
Keywords: delafloxacin; green RP-HPTLC; NP-HPTLC; ICH guidelines; solid lipid nanoparticles delafloxacin; green RP-HPTLC; NP-HPTLC; ICH guidelines; solid lipid nanoparticles
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MDPI and ACS Style

Alam, P.; Ezzeldin, E.; Iqbal, M.; Mostafa, G.A.E.; Anwer, M.K.; Alqarni, M.H.; Foudah, A.I.; Shakeel, F. Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study. Antibiotics 2020, 9, 359. https://doi.org/10.3390/antibiotics9060359

AMA Style

Alam P, Ezzeldin E, Iqbal M, Mostafa GAE, Anwer MK, Alqarni MH, Foudah AI, Shakeel F. Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study. Antibiotics. 2020; 9(6):359. https://doi.org/10.3390/antibiotics9060359

Chicago/Turabian Style

Alam, Prawez; Ezzeldin, Essam; Iqbal, Muzaffar; Mostafa, Gamal A.E.; Anwer, Md. K.; Alqarni, Mohammed H.; Foudah, Ahmed I.; Shakeel, Faiyaz. 2020. "Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study" Antibiotics 9, no. 6: 359. https://doi.org/10.3390/antibiotics9060359

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