Next Article in Journal
Potent and Specific Antibacterial Activity against Escherichia coli O157:H7 and Methicillin Resistant Staphylococcus aureus (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles
Next Article in Special Issue
Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications
Previous Article in Journal
Colonization of Methicillin-Resistant Staphylococcus aureus (MRSA) among Medical Students in Tertiary Institution in Central Malaysia
Previous Article in Special Issue
Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study
Open AccessArticle

Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data

1
Department of Chemistry, University of Zanjan, Zanjan 45195-313, Iran
2
Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium
3
Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo—Ourense Campus, E-32004 Ourense, Spain
*
Authors to whom correspondence should be addressed.
Antibiotics 2020, 9(7), 383; https://doi.org/10.3390/antibiotics9070383
Received: 5 June 2020 / Revised: 2 July 2020 / Accepted: 3 July 2020 / Published: 6 July 2020
(This article belongs to the Special Issue Challenges in Antibiotics Analysis)
The main objective of this study was to develop a simple and efficient spectrophotometric technique combined with chemometrics for the simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP) in drug formulations. Specifically, we sought: (i) to evaluate the potential use of rank annihilation factor analysis (RAFA) to pH gradual change spectrophotometric data in order to provide sufficient accuracy and model robustness; and (ii) to determine SMX and TMP concentration in drug formulations without tedious pre-treatments such as derivatization or extraction techniques which are time-consuming and require hazardous solvents. In the proposed method, the spectra of the sample solutions at different pH values were recorded and the pH-spectra bilinear data matrix was generated. On these data, RAFA was then applied to estimate the concentrations of SMX and TMP in synthetic and real samples. Applying RAFA showed that the two drugs could be determined simultaneously with concentration ratios of SMX to TMP varying from 1:30 to 30:1 in the mixed samples (concentration range is 1–30 µg mL−1 for both components). The limits of detection were 0.25 and 0.38 µg mL−1 for SMX and TMP, respectively. The proposed method was successfully applied to the simultaneous determination of SMX and TMP in some synthetic, pharmaceutical formulation and biological fluid samples. In addition, the means of the estimated RSD (%) were 1.71 and 2.18 for SMX and TMP, respectively, in synthetic mixtures. The accuracy of the proposed method was confirmed by spiked recovery test on biological samples with satisfactory results (90.50–109.80%). View Full-Text
Keywords: bilinear matrix; pH-spectra; rank annihilation factor analysis; Sulfamethoxazole; Trimethoprim bilinear matrix; pH-spectra; rank annihilation factor analysis; Sulfamethoxazole; Trimethoprim
Show Figures

Figure 1

MDPI and ACS Style

Esteki, M.; Dashtaki, E.; Heyden, Y.V.; Simal-Gandara, J. Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data. Antibiotics 2020, 9, 383. https://doi.org/10.3390/antibiotics9070383

AMA Style

Esteki M, Dashtaki E, Heyden YV, Simal-Gandara J. Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data. Antibiotics. 2020; 9(7):383. https://doi.org/10.3390/antibiotics9070383

Chicago/Turabian Style

Esteki, Mahnaz; Dashtaki, Elham; Heyden, Yvan V.; Simal-Gandara, Jesus. 2020. "Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data" Antibiotics 9, no. 7: 383. https://doi.org/10.3390/antibiotics9070383

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop