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Antibiotics
Volume 9
Issue 5
10.3390/antibiotics9050237
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Article

High Abundance of Proteobacteria in Ileo-Anal Pouch Anastomosis and Increased Abundance of Fusobacteria Associated with Increased Pouch Inflammation

by
Andreas Munk Petersen
1,2,3,*,
Hengameh Chloé Mirsepasi-Lauridsen
4,
Marianne K. Vester-Andersen
1,5,
Nikolaj Sørensen
6,
Karen Angeliki Krogfelt
4,7,8 and
Flemming Bendtsen
1,3
1
Gastrounit, Medical Section, Amager-Hvidovre University Hospital, 2650 Copenhagen, Denmark
2
Department of Clinical Microbiology, Amager-Hvidovre University Hospital, 2650 Copenhagen, Denmark
3
Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark
4
Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark
5
Department of Internal Medicine, Zealand University Hospital, 4600 Køge, Denmark
6
Clinical-Microbiomics, Ole Maaløes Vej 3, Clinical Microbiomics, 2200 Copenhagen, Denmark
7
Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, 2300 Copenhagen, Denmark
8
Department of Science and Environment, Roskilde University, 4000 Roskilde, Denmark
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(5), 237; https://doi.org/10.3390/antibiotics9050237
Submission received: 6 March 2020 / Revised: 3 May 2020 / Accepted: 6 May 2020 / Published: 8 May 2020
(This article belongs to the Special Issue Pathogenic Escherichia coli: Infections and Therapies)
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Abstract

Low diversity intestinal dysbiosis has been associated with inflammatory bowel disease, including patients with ulcerative colitis with an ileo-anal pouch anastomosis. Furthermore, specific Escherichia coli phylogroups have been linked to inflammatory bowel disease. Our aim was to characterize the differences among microbiota and E. coli phylogroups in active and inactive pouchitis. Disease activity was assessed using the modified pouch disease activity index and by fecal calprotectin. Microbiota diversity was assessed by 16S rDNA MiSeq sequencing. E. coli phylogroup was determined after triplex PCR. Twenty patients with ulcerative colitis with an ileo-anal pouch anastomosis were included, 10 of whom had active pouchitis. Ileo-anal pouch anastomosis patients had an increased abundance of Proteobacteria colonization compared to patients with ulcerative colitis or Crohn’s disease and healthy controls, p = 1.4·10−5. No differences in E. coli phylogroup colonization could be determined between cases of active and inactive disease. No significant link was found between α-diversity and pouch inflammation. However, higher levels of Fusobacteria colonization were found in patients with a pouch with a fecal calprotectin level above 500, p = 0.02. In conclusion, patients with a pouch had an increased Proteobacteria abundance, but only Fusobacteria abundance was linked to inflammation.
Keywords: Proteobacteria; Escherichia coli; Fusobacteria; pouchitis; inflammatory bowel disease; calprotectin Proteobacteria; Escherichia coli; Fusobacteria; pouchitis; inflammatory bowel disease; calprotectin

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MDPI and ACS Style

Petersen, A.M.; Mirsepasi-Lauridsen, H.C.; Vester-Andersen, M.K.; Sørensen, N.; Krogfelt, K.A.; Bendtsen, F. High Abundance of Proteobacteria in Ileo-Anal Pouch Anastomosis and Increased Abundance of Fusobacteria Associated with Increased Pouch Inflammation. Antibiotics 2020, 9, 237. https://doi.org/10.3390/antibiotics9050237

AMA Style

Petersen AM, Mirsepasi-Lauridsen HC, Vester-Andersen MK, Sørensen N, Krogfelt KA, Bendtsen F. High Abundance of Proteobacteria in Ileo-Anal Pouch Anastomosis and Increased Abundance of Fusobacteria Associated with Increased Pouch Inflammation. Antibiotics. 2020; 9(5):237. https://doi.org/10.3390/antibiotics9050237

Chicago/Turabian Style

Petersen, Andreas Munk, Hengameh Chloé Mirsepasi-Lauridsen, Marianne K. Vester-Andersen, Nikolaj Sørensen, Karen Angeliki Krogfelt, and Flemming Bendtsen. 2020. "High Abundance of Proteobacteria in Ileo-Anal Pouch Anastomosis and Increased Abundance of Fusobacteria Associated with Increased Pouch Inflammation" Antibiotics 9, no. 5: 237. https://doi.org/10.3390/antibiotics9050237

APA Style

Petersen, A. M., Mirsepasi-Lauridsen, H. C., Vester-Andersen, M. K., Sørensen, N., Krogfelt, K. A., & Bendtsen, F. (2020). High Abundance of Proteobacteria in Ileo-Anal Pouch Anastomosis and Increased Abundance of Fusobacteria Associated with Increased Pouch Inflammation. Antibiotics, 9(5), 237. https://doi.org/10.3390/antibiotics9050237

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