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Open AccessArticle

Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys

1
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
2
Department of Microbiology and Public Health, Khulna Agricultural University, Khulna 9208, Bangladesh
3
Department of Integrative Biology, College of Arts and Sciences, University of South Florida, St. Petersburg, FL 33701, USA
4
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
*
Authors to whom correspondence should be addressed.
Antibiotics 2020, 9(11), 770; https://doi.org/10.3390/antibiotics9110770
Received: 23 August 2020 / Revised: 21 October 2020 / Accepted: 25 October 2020 / Published: 2 November 2020
(This article belongs to the Special Issue Antimicrobial Resistance and Virulence Mechanisms)
Diseases caused by Escherichia coli (E. coli) and Salmonella spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) E. coli and Salmonella spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene tetA was detected by PCR. All samples were positive for E. coli. Only 11 samples (11/30; 36.67%) were positive for Salmonella spp. from healthy turkeys, whereas 16 (16/25; 64%) samples were positive for Salmonella spp. from diseased turkeys. E. coli isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. Salmonella spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All E. coli and Salmonella spp. from both healthy and diseased turkeys were resistant to erythromycin. Salmonella spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both E. coli and Salmonella spp. from diseased turkeys. Finally, the tetA gene was detected in 93.1% of the E. coli isolates and in 92.59% of the Salmonella spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize tetA-gene-containing MDR E. coli and Salmonella spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge. View Full-Text
Keywords: avian colibacillosis; salmonellosis; antibiotic resistance; MDR; tetA; public health avian colibacillosis; salmonellosis; antibiotic resistance; MDR; tetA; public health
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MDPI and ACS Style

Tawyabur, M..; Islam, M..S.; Sobur, M..A.; Hossain, M..J.; Mahmud, M..M.; Paul, S.; Hossain, M.T.; Ashour, H.M.; Rahman, M..T. Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys. Antibiotics 2020, 9, 770.

AMA Style

Tawyabur M, Islam MS, Sobur MA, Hossain MJ, Mahmud MM, Paul S, Hossain MT, Ashour HM, Rahman MT. Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys. Antibiotics. 2020; 9(11):770.

Chicago/Turabian Style

Tawyabur, Md.; Islam, Md. S.; Sobur, Md. A.; Hossain, Md. J.; Mahmud, Md. M.; Paul, Sumon; Hossain, Muhammad T.; Ashour, Hossam M.; Rahman, Md. T. 2020. "Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys" Antibiotics 9, no. 11: 770.

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