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Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
Open AccessArticle

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

1
Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Universidad Industrial de Santander, Bucaramanga 680002, Colombia
2
Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, Colombia
3
Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga 680002, Colombia
4
Centro de Cromatografía y Espectrometría de Masas, CROM-MASS-CENIVAM, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga 680002, Colombia
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(11), 730; https://doi.org/10.3390/antibiotics9110730
Received: 15 September 2020 / Revised: 6 October 2020 / Accepted: 23 October 2020 / Published: 24 October 2020
(This article belongs to the Special Issue Synthesis and Pharmacokinetics of Antibiotics)
The emergence of multidrug resistant microorganisms represents a global challenge due to the lack of new effective antimicrobial agents. In this sense, essential oils (EOs) are an alternative to be considered because of their anti-inflammatory, antiviral, antibacterial, and antibiofilm biological activities. Therefore, multiple efforts have been made to consider the potential use of EOs in the treatment of infections which are caused by resistant microorganisms. In this study, 15 EOs of both Colombian and introduced aromatic plants were evaluated against pathogenic strains of E. coli O157:H7 and methicillin resistant Staphylococcus aureus (MRSA) in planktonic and sessile states in order to identify relevant and promising alternatives for the treatment of microbial infections. Forty different compounds were identified in the 15 EO with nine of them constituted mainly by oxygenated monoterpenes (OM). EOs from Lippia origanoides, chemotypes thymol, and carvacrol, displayed the highest antibacterial activity against E. coli O157:H7 (MIC50 = 0.9 and 0.3 mg/mL, respectively) and MRSA (MIC50 = 1.2 and 0.6 mg/mL, respectively). These compounds from EOs had also the highest antibiofilm activity (inhibition percentage > 70.3%). Using scanning electron microscopy (SEM), changes in the size and morphology of both bacteria were observed when they were exposed to sub-inhibitory concentrations of L. origanoides EO carvacrol chemotype. EOs from L. origanoides, thymol, and carvacrol chemotypes represented a viable alternative for the treatment of microbial infections; however, the Selectivity Index (SI ≤ 3) indicated that it was necessary to study alternatives to reduce its in vitro cytotoxicity. View Full-Text
Keywords: essential oil; MRSA; E. coli O157:H7; antibiofilm activity; natural antimicrobial compound; Lippia origanoides essential oil; MRSA; E. coli O157:H7; antibiofilm activity; natural antimicrobial compound; Lippia origanoides
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MDPI and ACS Style

Gómez-Sequeda, N.; Cáceres, M.; Stashenko, E.E.; Hidalgo, W.; Ortiz, C. Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA). Antibiotics 2020, 9, 730. https://doi.org/10.3390/antibiotics9110730

AMA Style

Gómez-Sequeda N, Cáceres M, Stashenko EE, Hidalgo W, Ortiz C. Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA). Antibiotics. 2020; 9(11):730. https://doi.org/10.3390/antibiotics9110730

Chicago/Turabian Style

Gómez-Sequeda, Nicolás; Cáceres, Marlon; Stashenko, Elena E.; Hidalgo, William; Ortiz, Claudia. 2020. "Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)" Antibiotics 9, no. 11: 730. https://doi.org/10.3390/antibiotics9110730

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