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Antibiotics 2018, 7(2), 31; https://doi.org/10.3390/antibiotics7020031

Complementation Studies of Bacteriophage λ O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction Phenotypes

Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada
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Received: 27 February 2018 / Revised: 25 March 2018 / Accepted: 3 April 2018 / Published: 5 April 2018
(This article belongs to the Special Issue Bacterial DNA Replication and Replication Inhibitors)
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Abstract

λ genes O and P are required for replication initiation from the bacteriophage λ origin site, oriλ, located within gene O. Questions have persisted for years about whether O-defects can indeed be complemented in trans. We show the effect of original null mutations in O and the influence of four origin mutations (three are in-frame deletions and one is a point mutation) on complementation. This is the first demonstration that O proteins with internal deletions can complement for O activity, and that expression of the N-terminal portion of gene P can completely prevent O complementation. We show that O-P co-expression can limit the lethal effect of P on cell growth. We explore the influence of the contiguous small RNA OOP on O complementation and P-lethality. View Full-Text
Keywords: bacteriophage lambda (λ); bi-directional replication initiation from oriλ; O and P initiator proteins; oriλ interaction site; O complementation by oriλ-defective alleles; influence of O:P interactions on cell growth and O activity bacteriophage lambda (λ); bi-directional replication initiation from oriλ; O and P initiator proteins; oriλ interaction site; O complementation by oriλ-defective alleles; influence of O:P interactions on cell growth and O activity
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Hayes, S.; Rajamanickam, K.; Hayes, C. Complementation Studies of Bacteriophage λ O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction Phenotypes. Antibiotics 2018, 7, 31.

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