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Open AccessFeature PaperArticle

Biosynthesis of Rishirilide B

1
Pharmaceutical Biology and Biotechnology, University of Freiburg, Stefan-Meier-Str. 19, 79104 Freiburg im Breisgau, Germany
2
Organic Chemistry, University of Siegen, Adolf-Reichwein-Str. 2, 57068 Siegen, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antibiotics 2018, 7(1), 20; https://doi.org/10.3390/antibiotics7010020
Received: 14 February 2018 / Revised: 1 March 2018 / Accepted: 1 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Actinomycetes: The Antibiotics Producers)
Rishirilide B was isolated from Streptomyces rishiriensis and Streptomyces bottropensis on the basis of its inhibitory activity towards alpha-2-macroglobulin. The biosynthesis of rishirilide B was investigated by feeding experiments with different 13C labelled precursors using the heterologous host Streptomyces albus J1074::cos4 containing a cosmid encoding of the gene cluster responsible for rishirilide B production. NMR spectroscopic analysis of labelled compounds demonstrate that the tricyclic backbone of rishirilide B is a polyketide synthesized from nine acetate units. One of the acetate units is decarboxylated to give a methyl group. The origin of the starter unit was determined to be isobutyrate. View Full-Text
Keywords: streptomyces; rishirilide; biosynthesis; polyketides streptomyces; rishirilide; biosynthesis; polyketides
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MDPI and ACS Style

Schwarzer, P.; Wunsch-Palasis, J.; Bechthold, A.; Paululat, T. Biosynthesis of Rishirilide B. Antibiotics 2018, 7, 20.

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