Biosynthesis of Rishirilide B
AbstractRishirilide B was isolated from Streptomyces rishiriensis and Streptomyces bottropensis on the basis of its inhibitory activity towards alpha-2-macroglobulin. The biosynthesis of rishirilide B was investigated by feeding experiments with different 13C labelled precursors using the heterologous host Streptomyces albus J1074::cos4 containing a cosmid encoding of the gene cluster responsible for rishirilide B production. NMR spectroscopic analysis of labelled compounds demonstrate that the tricyclic backbone of rishirilide B is a polyketide synthesized from nine acetate units. One of the acetate units is decarboxylated to give a methyl group. The origin of the starter unit was determined to be isobutyrate. View Full-Text
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Schwarzer, P.; Wunsch-Palasis, J.; Bechthold, A.; Paululat, T. Biosynthesis of Rishirilide B. Antibiotics 2018, 7, 20.
Schwarzer P, Wunsch-Palasis J, Bechthold A, Paululat T. Biosynthesis of Rishirilide B. Antibiotics. 2018; 7(1):20.Chicago/Turabian Style
Schwarzer, Philipp; Wunsch-Palasis, Julia; Bechthold, Andreas; Paululat, Thomas. 2018. "Biosynthesis of Rishirilide B." Antibiotics 7, no. 1: 20.
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