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Antibiotics 2018, 7(1), 20; https://doi.org/10.3390/antibiotics7010020

Biosynthesis of Rishirilide B

1
Pharmaceutical Biology and Biotechnology, University of Freiburg, Stefan-Meier-Str. 19, 79104 Freiburg im Breisgau, Germany
2
Organic Chemistry, University of Siegen, Adolf-Reichwein-Str. 2, 57068 Siegen, Germany
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 14 February 2018 / Revised: 1 March 2018 / Accepted: 1 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Actinomycetes: The Antibiotics Producers)
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Abstract

Rishirilide B was isolated from Streptomyces rishiriensis and Streptomyces bottropensis on the basis of its inhibitory activity towards alpha-2-macroglobulin. The biosynthesis of rishirilide B was investigated by feeding experiments with different 13C labelled precursors using the heterologous host Streptomyces albus J1074::cos4 containing a cosmid encoding of the gene cluster responsible for rishirilide B production. NMR spectroscopic analysis of labelled compounds demonstrate that the tricyclic backbone of rishirilide B is a polyketide synthesized from nine acetate units. One of the acetate units is decarboxylated to give a methyl group. The origin of the starter unit was determined to be isobutyrate. View Full-Text
Keywords: streptomyces; rishirilide; biosynthesis; polyketides streptomyces; rishirilide; biosynthesis; polyketides
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Schwarzer, P.; Wunsch-Palasis, J.; Bechthold, A.; Paululat, T. Biosynthesis of Rishirilide B. Antibiotics 2018, 7, 20.

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